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PMID: 15534003 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Live cell imaging of the assembly, disassembly, and actin cable-dependent movement of endosomes and actin patches in the budding yeast, Saccharomyces cerevisiae.

The Journal of cell biology ·Vol. 167 ·No. 3 ·2004-11-08 ·Pages 519-30

Huckaba TM, Gay AC, Pantalena LF, Yang HC, Pon LA

Abstract

Using FM4-64 to label endosomes and Abp1p-GFP or Sac6p-GFP to label actin patches, we find that (1) endosomes colocalize with actin patches as they assemble at the bud cortex; (2) endosomes colocalize with actin patches as they undergo linear, retrograde movement from buds toward mother cells; and (3) actin patches interact with and disassemble at FM4-64-labeled internal compartments. We also show that retrograde flow of actin cables mediates retrograde actin patch movement. An Arp2/3 complex mutation decreases the frequency of cortical, nonlinear actin patch movements, but has no effect on the velocity of linear, retrograde actin patch movement. Rather, linear actin patch movement occurs at the same velocity and direction as the movement of actin cables. Moreover, actin patches require actin cables for retrograde movements and colocalize with actin cables as they undergo retrograde movement. Our studies support a mechanism whereby actin cables serve as "conveyor belts" for retrograde movement and delivery of actin patches/endosomes to FM4-64-labeled internal compartments.

MeSH Terms
Actin-Related Protein 2 Actin-Related Protein 3 Actins/metabolism,physiology Cell Compartmentation Endocytosis Endosomes/metabolism,physiology Macromolecular Substances Movement Protein Binding Protein Transport Saccharomyces cerevisiae/cytology,ultrastructure Saccharomyces cerevisiae Proteins/metabolism,physiology
Chemicals
ARP2 protein, S cerevisiae ARP3 protein, S cerevisiae Actin-Related Protein 2 Actin-Related Protein 3 Actins Macromolecular Substances Saccharomyces cerevisiae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Huckaba Thomas M
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
Gay Anna Card
Pantalena Luiz Fernando
Yang Hyeong-Cheol
Pon Liza A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200404173
Published
2004-11-08
Pages
519-30
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2172478
Subset
IM
Grants
NIGMS NIH HHS · R01 GM045735 · United States
NIDDK NIH HHS · DDK07786 · United States
NIGMS NIH HHS · GM45735 · United States
NIGMS NIH HHS · R01 GM066307 · United States
NIGMS NIH HHS · GM66307 · United States
NIDDK NIH HHS · T32 DK007786 · United States
External Links
PubMed source DOI: 10.1083/jcb.200404173 PMC Full Text View journal details
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