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PMID: 7812049 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Yeast mitochondria contain ATP-sensitive, reversible actin-binding activity.

Molecular biology of the cell ·Vol. 5 ·No. 7 ·1994-07-00 ·Pages 807-18

Lazzarino DA, Boldogh I, Smith MG, Rosand J, Pon LA

Abstract

Sedimentation assays were used to demonstrate and characterize binding of isolated yeast mitochondria to phalloidin-stabilized yeast F-actin. These actin-mitochondrial interactions are ATP sensitive, saturable, reversible, and do not depend upon mitochondrial membrane potential. Protease digestion of mitochondrial outer membrane proteins or saturation of myosin-binding sites on F-actin with the S1 subfragment of skeletal myosin block binding. These observations indicate that a protein (or proteins) on the mitochondrial surface mediates ATP-sensitive, reversible binding of mitochondria to the lateral surface of microfilaments. Actin copurifies with mitochondria during subcellular fractionation and is released from the organelle upon treatment with ATP. Thus, actin-mitochondrial interactions resembling those observed in vitro may also exist in intact yeast cells. Finally, a yeast mutant bearing a temperature-sensitive mutation in the actin-encoding ACT1 gene (act1-3) displays temperature-dependent defects in transfer of mitochondria from mother cells to newly developed buds during yeast cell mitosis.

Related Genes
ACT1
MeSH Terms
Actins/genetics,metabolism Adenosine Triphosphate/pharmacology Fungal Proteins/metabolism Microfilament Proteins/genetics,metabolism Mitochondria/metabolism Myosins/metabolism Point Mutation Protein Binding/drug effects Saccharomyces cerevisiae/genetics,metabolism Ultracentrifugation
Chemicals
Actins Fungal Proteins Microfilament Proteins Adenosine Triphosphate Myosins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lazzarino D A
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Boldogh I
Smith M G
Rosand J
Pon L A
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.5.7.807
Published
1994-07-00
Pages
807-18
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC301098
Subset
IM
Grants
NINDS NIH HHS · 2T32 NS-07062 · United States
NHLBI NIH HHS · 5T35 HL-07616 · United States
NCRR NIH HHS · SO7 RR-05395 · United States
External Links
PubMed source DOI: 10.1091/mbc.5.7.807 PMC Full Text View journal details
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