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PMID: 11248049 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Arp2/3 complex and actin dynamics are required for actin-based mitochondrial motility in yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 6 ·2001-03-13 ·Pages 3162-7

Boldogh IR, Yang HC, Nowakowski WD, Karmon SL, Hays LG, Yates JR, Pon LA

Abstract

The Arp2/3 complex is implicated in actin polymerization-driven movement of Listeria monocytogenes. Here, we find that Arp2p and Arc15p, two subunits of this complex, show tight, actin-independent association with isolated yeast mitochondria. Arp2p colocalizes with mitochondria. Consistent with this result, we detect Arp2p-dependent formation of actin clouds around mitochondria in intact yeast. Cells bearing mutations in ARP2 or ARC15 genes show decreased velocities of mitochondrial movement, loss of all directed movement and defects in mitochondrial morphology. Finally, we observe a decrease in the velocity and extent of mitochondrial movement in yeast in which actin dynamics are reduced but actin cytoskeletal structure is intact. These results support the idea that the movement of mitochondria in yeast is actin polymerization driven and that this movement requires Arp2/3 complex.

MeSH Terms
Actin-Related Protein 2 Actin-Related Protein 3 Actins/metabolism Cytoskeletal Proteins Fungal Proteins/metabolism Membrane Proteins/metabolism Mitochondria/metabolism,physiology Potassium Chloride/metabolism,pharmacology Saccharomyces cerevisiae/drug effects,metabolism
Chemicals
Actin-Related Protein 2 Actin-Related Protein 3 Actins Cytoskeletal Proteins Fungal Proteins Membrane Proteins Potassium Chloride
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Boldogh I R
Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032, USA.
Yang H C
Nowakowski W D
Karmon S L
Hays L G
Yates J R
Pon L A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.051494698
Published
2001-03-13
Pages
3162-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC30624
Subset
IM
Grants
NIGMS NIH HHS · R01 GM045735 · United States
NCI NIH HHS · P30CA13696 · United States
NIGMS NIH HHS · GM45735 · United States
NCRR NIH HHS · S10RR10506 · United States
NCI NIH HHS · P30 CA013696 · United States
NCRR NIH HHS · RR11832 · United States
External Links
PubMed source DOI: 10.1073/pnas.051494698 PMC Full Text View journal details
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