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PMID: 22045228 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Wobble base-pairing slows in vivo translation elongation in metazoans.

RNA (New York, N.Y.) ·Vol. 17 ·No. 12 ·2011-12-00 ·Pages 2063-73

Stadler M, Fire A

Abstract

In the universal genetic code, most amino acids can be encoded by multiple trinucleotide codons, and the choice among available codons can influence position-specific translation elongation rates. By using sequence-based ribosome profiling, we obtained transcriptome-wide profiles of in vivo ribosome occupancy as a function of codon identity in Caenorhabditis elegans and human cells. Particularly striking in these profiles was a universal trend of higher ribosome occupancy for codons translated via G:U wobble base-pairing compared with synonymous codons that pair with the same tRNA family using G:C base-pairing. These data support a model in which ribosomal translocation is slowed at wobble codon positions.

MeSH Terms
Animals Anticodon/chemistry Base Pairing Base Sequence Binding Sites Caenorhabditis elegans/genetics,metabolism Codon/chemistry Gene Expression Profiling Gene Expression Regulation HeLa Cells Humans Peptide Chain Elongation, Translational/genetics RNA, Messenger/metabolism RNA, Transfer/metabolism Ribosomes/genetics,metabolism
Chemicals
Anticodon Codon RNA, Messenger RNA, Transfer
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stadler Michael
Department of Genetics, Stanford University, Stanford, California 94305-5324, USA.
Fire Andrew
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1469-9001
DOI
10.1261/rna.02890211
Published
2011-12-00
Epub
2011-00-01
Pages
2063-73
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC3222120
Subset
IM
Grants
NIGMS NIH HHS · R01 GM037706 · United States
NIGMS NIH HHS · R01GM37706 · United States
PHS HHS · U54065359 · United States
NICHD NIH HHS · T32-HD00044 · United States
External Links
PubMed source DOI: 10.1261/rna.02890211 PMC Full Text View journal details
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