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PMID: 2204817 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cell type-specific mechanisms of regulating expression of the ornithine decarboxylase gene after growth stimulation.

Molecular and cellular biology ·Vol. 10 ·No. 10 ·1990-10-00 ·Pages 5525-8

Abrahamsen MS, Morris DR

Abstract

Ornithine decarboxylase (ODC) mRNA is strongly induced by mitogenic activation of resting Swiss 3T3 fibroblasts and T lymphocytes. Nuclear run-on analysis revealed a low level of nascent transcripts in resting fibroblasts that was elevated upon activation. In contrast, there was a high level of transcription across the entire ODC gene in resting T cells, which remained unchanged upon activation. The stability of the mature ODC message was found to be unaffected by mitogenic stimulation. These results indicate that ODC mRNA levels are regulated transcriptionally in Swiss 3T3 cells and posttranscriptionally within the nucleus of T lymphocytes in response to mitogenic stimuli. In this unique situation, the mitogenic induction of a single gene, ODC, is regulated by two very distinct, cell-specific mechanisms.

MeSH Terms
Animals Cattle Cell Division Cell Nucleus/physiology Colforsin/pharmacology Fibroblasts/physiology Gene Expression Regulation, Enzymologic In Vitro Techniques Mice Ornithine Decarboxylase/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-myc Proto-Oncogenes RNA, Messenger/genetics T-Lymphocytes/physiology Tetradecanoylphorbol Acetate/pharmacology Transcription, Genetic
Chemicals
Proto-Oncogene Proteins Proto-Oncogene Proteins c-myc RNA, Messenger Colforsin Ornithine Decarboxylase Tetradecanoylphorbol Acetate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Abrahamsen M S
Department of Biochemistry, University of Washington, Seattle 98195.
Morris D R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.10.5525-5528.1990
Published
1990-10-00
Pages
5525-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361267
Subset
IM
Grants
NCI NIH HHS · CA39053 · United States
NIDCR NIH HHS · DE08229 · United States
NIGMS NIH HHS · GM07270 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.10.5525-5528.1990 PMC Full Text View journal details
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