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PMID: 22073207 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Carbon dynamics, development and stress responses in Arabidopsis: involvement of the APL4 subunit of ADP-glucose pyrophosphorylase (starch synthesis).

PloS one ·Vol. 6 ·No. 11 ·2011-00-00 ·Pages e26855

Sulmon C, Gouesbet G, Ramel F, Cabello-Hurtado F, Penno C, Bechtold N, Couée I, El Amrani A

Abstract

An Arabidopsis thaliana T-DNA insertional mutant was identified and characterized for enhanced tolerance to the singlet-oxygen-generating herbicide atrazine in comparison to wild-type. This enhanced atrazine tolerance mutant was shown to be affected in the promoter structure and in the regulation of expression of the APL4 isoform of ADP-glucose pyrophosphorylase, a key enzyme of the starch biosynthesis pathway, thus resulting in decrease of APL4 mRNA levels. The impact of this regulatory mutation was confirmed by the analysis of an independent T-DNA insertional mutant also affected in the promoter of the APL4 gene. The resulting tissue-specific modifications of carbon partitioning in plantlets and the effects on plantlet growth and stress tolerance point out to specific and non-redundant roles of APL4 in root carbon dynamics, shoot-root relationships and sink regulations of photosynthesis. Given the effects of exogenous sugar treatments and of endogenous sugar levels on atrazine tolerance in wild-type Arabidopsis plantlets, atrazine tolerance of this apl4 mutant is discussed in terms of perception of carbon status and of investment of sugar allocation in xenobiotic and oxidative stress responses.

MeSH Terms
Arabidopsis/enzymology,genetics,metabolism Atrazine/pharmacology Carbon/metabolism DNA, Bacterial/genetics Glucose-1-Phosphate Adenylyltransferase/chemistry,metabolism Herbicides/pharmacology Molecular Sequence Data Mutagenesis, Insertional Promoter Regions, Genetic
Chemicals
DNA, Bacterial Herbicides T-DNA Carbon Glucose-1-Phosphate Adenylyltransferase Atrazine
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Sulmon Cécile
Centre National de la Recherche Scientifique, Université de Rennes 1, UMR 6553 ECOBIO, Rennes, France. [email protected]
Gouesbet Gwenola
Ramel Fanny
Cabello-Hurtado Francisco
Penno Christophe
Bechtold Nicole
Couée Ivan
El Amrani Abdelhak
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0026855
Email
[email protected]
Published
2011-00-00
Epub
2011-00-03
Pages
e26855
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3207819
Subset
IM
Databases
GENBANK
AY059075, AY084978
External Links
PubMed source DOI: 10.1371/journal.pone.0026855 PMC Full Text View journal details
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