Home LiteratureArticle Details
PMID: 2211522 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The ClpP component of Clp protease is the sigma 32-dependent heat shock protein F21.5.

Journal of bacteriology ·Vol. 172 ·No. 10 ·1990-10-00 ·Pages 6026-34

Kroh HE, Simon LD

Abstract

The genes that encode the subunits of the Clp protease of Escherichia coli, clpA and clpP, appear to be regulated differently from each other. The clpA gene does not seem to be under heat shock control (Y. S. Katayama, S. Gottesman, J. Pumphrey, S. Rudikoff, W. P. Clark, and M. R. Maurizi, J. Biol. Chem. 263:15226-15236, 1988). In contrast, the level of ClpP protein was increased in rpoH+ cells but not in null rpoH cells after an upshift in temperature from 17 to 43 degrees C. The level of ClpP protein in a null dnaK strain was also elevated relative to the level of ClpP protein in an otherwise isogenic dnaK+ strain. In two-dimensional gels, the ClpP protein was located in the position of the previously unidentified heat shock protein F21.5. No protein spot corresponding to F21.5 was present in two-dimensional gels of a null clpP strain. The clpP gene, therefore, appears to be a heat shock gene, expressed in a sigma 32-dependent manner and negatively regulated by DnaK; the product of clpP is the previously unidentified heat shock protein F21.5.

MeSH Terms
ATP-Dependent Proteases Electrophoresis, Gel, Two-Dimensional Escherichia coli/enzymology,genetics Genes, Bacterial Genotype Heat-Shock Proteins/biosynthesis,genetics,isolation & purification Macromolecular Substances Promoter Regions, Genetic Recombinant Fusion Proteins/biosynthesis,isolation & purification Serine Endopeptidases/biosynthesis,genetics,isolation & purification Sigma Factor/metabolism
Chemicals
Heat-Shock Proteins Macromolecular Substances Recombinant Fusion Proteins Sigma Factor ATP-Dependent Proteases Serine Endopeptidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kroh H E
Waksman Institute, Rutgers, State University of New Jersey, Piscataway, New Jersey 08854.
Simon L D
References (47)
47 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. The (A)BC excinuclease of Escherichia coli has only the UvrB and UvrC subunits in the incision complex.
    Proc Natl Acad Sci U S A. 1989 Jul;86(14):5237-41 PMID: 2546148
  3. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  4. Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates.
    Cell. 1978 May;14(1):179-90 PMID: 352533
  5. Identity of the B56.5 protein, the A-protein, and the groE gene product of Escherichia coli.
    J Bacteriol. 1981 Jan;145(1):513-20 PMID: 6161915
  6. Multiple forms of lysyl-transfer ribonucleic acid synthetase in Escherichia coli.
    J Bacteriol. 1981 Apr;146(1):345-51 PMID: 7012120
  7. Identification and purification of the Lon+ (capR+) gene product, a DNA-binding protein.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2043-7 PMID: 6264459
  8. E. coli contains eight soluble proteolytic activities, one being ATP dependent.
    Nature. 1981 Aug 13;292(5824):652-4 PMID: 7019728
  9. The structure and function of the regulatory elements of the Escherichia coli uvrB gene.
    Nucleic Acids Res. 1981 Nov 11;9(21):5623-43 PMID: 6273801
  10. The B66.0 protein of Escherichia coli is the product of the dnaK+ gene.
    J Bacteriol. 1982 Mar;149(3):1175-7 PMID: 7037741
  11. Genetic control of heat-shock protein synthesis and its bearing on growth and thermal resistance in Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1982 Feb;79(3):860-4 PMID: 7038687
  12. Protein degradation in Escherichia coli: the lon gene controls the stability of sulA protein.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):358-62 PMID: 6300834
  13. Identification of the heat-inducible protein C15.4 as the groES gene product in Escherichia coli.
    J Bacteriol. 1983 Jun;154(3):1505-7 PMID: 6343358
  14. A common regulatory region shared by divergently transcribed genes of the Escherichia coli SOS system.
    Nature. 1983 Sep 15-21;305(5931):243-5 PMID: 6310417
  15. The dnaK protein modulates the heat-shock response of Escherichia coli.
    Cell. 1983 Sep;34(2):641-6 PMID: 6311435
  16. Regulation of the uvrC gene of Escherichia coli K12: localization and characterization of a damage-inducible promoter.
    EMBO J. 1983;2(12):2313-8 PMID: 6321159
  17. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.
    Microbiol Rev. 1984 Mar;48(1):60-93 PMID: 6371470
  18. lon gene product of Escherichia coli is a heat-shock protein.
    J Bacteriol. 1984 Jul;159(1):283-7 PMID: 6330035
  19. A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region.
    Cell. 1983 May;33(1):249-60 PMID: 6380755
  20. Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymerase.
    Cell. 1984 Sep;38(2):371-81 PMID: 6380764
  21. The htpR gene product of E. coli is a sigma factor for heat-shock promoters.
    Cell. 1984 Sep;38(2):383-90 PMID: 6380765
  22. Heat shock regulatory gene htpR influences rates of protein degradation and expression of the lon gene in Escherichia coli.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6647-51 PMID: 6436819
  23. A gene regulating the heat shock response in Escherichia coli also affects proteolysis.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6779-83 PMID: 6387713
  24. The genetics and regulation of heat-shock proteins.
    Annu Rev Genet. 1984;18:295-329 PMID: 6442118
  25. Production of abnormal proteins in E. coli stimulates transcription of lon and other heat shock genes.
    Cell. 1985 Jun;41(2):587-95 PMID: 3886165
  26. Optimizing the expression in E. coli of a synthetic gene encoding somatomedin-C (IGF-I).
    Nucleic Acids Res. 1985 Mar 25;13(6):1923-38 PMID: 3889844
  27. The role of ATP hydrolysis in the breakdown of proteins and peptides by protease La from Escherichia coli.
    J Biol Chem. 1985 Oct 5;260(22):12029-34 PMID: 2931432
  28. Insertional mutagenesis of the lon gene in Escherichia coli: lon is dispensable.
    J Bacteriol. 1985 Dec;164(3):1124-35 PMID: 2999072
  29. The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein.
    J Biol Chem. 1986 Feb 5;261(4):1782-5 PMID: 3003085
  30. Escherichia coli grpE gene codes for heat shock protein B25.3, essential for both lambda DNA replication at all temperatures and host growth at high temperature.
    J Bacteriol. 1986 Jul;167(1):25-9 PMID: 2424889
  31. Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli.
    J Bacteriol. 1987 Jan;169(1):26-32 PMID: 3539918
  32. Escherichia coli dnaK null mutants are inviable at high temperature.
    J Bacteriol. 1987 Jan;169(1):283-90 PMID: 3025174
  33. A multiple-component, ATP-dependent protease from Escherichia coli.
    J Biol Chem. 1987 Apr 5;262(10):4477-85 PMID: 3549708
  34. Escherichia coli contains a soluble ATP-dependent protease (Ti) distinct from protease La.
    Proc Natl Acad Sci U S A. 1987 Aug;84(16):5550-4 PMID: 3303028
  35. Induction of the heat shock regulon does not produce thermotolerance in Escherichia coli.
    Genes Dev. 1987 Aug;1(6):525-31 PMID: 3315852
  36. Divergent effects of a dnaK mutation on abnormal protein degradation in Escherichia coli.
    Mol Microbiol. 1988 Jan;2(1):31-41 PMID: 3130542
  37. Protease Ti, a new ATP-dependent protease in Escherichia coli, contains protein-activated ATPase and proteolytic functions in distinct subunits.
    J Biol Chem. 1988 Jun 25;263(18):8727-34 PMID: 2967816
  38. Isolation and characterization of Escherichia coli mutants that lack the heat shock sigma factor sigma 32.
    J Bacteriol. 1988 Aug;170(8):3640-9 PMID: 2900239
  39. Sequence of the lon gene in Escherichia coli. A heat-shock gene which encodes the ATP-dependent protease La.
    J Biol Chem. 1988 Aug 25;263(24):11718-28 PMID: 3042779
  40. Sequence analysis and transcriptional regulation of the Escherichia coli grpE gene, encoding a heat shock protein.
    Nucleic Acids Res. 1988 Aug 11;16(15):7545-62 PMID: 3045760
  41. Incision of damaged versus nondamaged DNA by the Escherichia coli UvrABC proteins.
    Nucleic Acids Res. 1988 Aug 25;16(16):7855-65 PMID: 2843804
  42. The two-component, ATP-dependent Clp protease of Escherichia coli. Purification, cloning, and mutational analysis of the ATP-binding component.
    J Biol Chem. 1988 Oct 15;263(29):15226-36 PMID: 3049606
  43. Characterization of the helicase activity of the Escherichia coli UvrAB protein complex.
    J Biol Chem. 1989 Jan 15;264(2):1336-43 PMID: 2536020
  44. Induction of a heat shock-like response by unfolded protein in Escherichia coli: dependence on protein level not protein degradation.
    Genes Dev. 1989 Aug;3(8):1226-32 PMID: 2676724
  45. Escherichia coli heat shock gene mutants are defective in proteolysis.
    Genes Dev. 1988 Dec;2(12B):1851-8 PMID: 3149251
  46. The heat-shock-regulated grpE gene of Escherichia coli is required for bacterial growth at all temperatures but is dispensable in certain mutant backgrounds.
    J Bacteriol. 1989 May;171(5):2748-55 PMID: 2651417
  47. Radioactive labeling of proteins in vitro.
    J Biol Chem. 1971 Feb 10;246(3):831-2 PMID: 5100767
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.10.6026-6034.1990
Published
1990-10-00
Pages
6026-34
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC526925
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.10.6026-6034.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2