Home LiteratureArticle Details
PMID: 6273801 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The structure and function of the regulatory elements of the Escherichia coli uvrB gene.

Nucleic acids research ·Vol. 9 ·No. 21 ·1981-11-11 ·Pages 5623-43

van den Berg E, Zwetsloot J, Noordermeer I, Pannekoek H, Dekker B, Dijkema R, van Ormondt H

Abstract

The construction and properties of recombinant plasmids carrying the Escherichia coli uvrB gene, including its transcriptional- and translational regulatory elements, is reported. The DNA sequence of the region, which governs the expression of the uvrB gene, has been determined. Within this sequence two non-overlapping DNA segments match the model sequence for Escherichia coli promoters (1). The '-10 regions' and the '-35 regions' of the proposed uvrB promoters are, respectively, 5'TAAAAT (P1), 5'TATAAT (P2) and 5'TTGGCA (P1), 5'GTGATG (P2). The existence and the position of these promoters has been established by elimination of one promoter (P2), using molecular cloning procedures, by length measurements of in vitro synthesized 'run-off' transcripts and by protection of the uvrB regulatory region for S1 nuclease digestion using in vivo made RNA. Potential sites of interaction within the uvrB regulatory region with regulatory proteins, such as the LexA protein (2) and the UvrC protein (3) are discussed.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Escherichia coli/genetics Genes, Regulator Plasmids Protein Biosynthesis Recombination, Genetic Transcription, Genetic
Chemicals
DNA, Recombinant DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
van den Berg E
Zwetsloot J
Noordermeer I
Pannekoek H
Dekker B
Dijkema R
van Ormondt H
References (45)
45 references, click to expand
  1. Expression of the cloned uvrB gene of Escherichia coli: dependency on nonsense suppressors.
    J Bacteriol. 1979 Jul;139(1):48-53 PMID: 378960
  2. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
    J Biol Chem. 1969 Nov 25;244(22):6160-7 PMID: 4900510
  3. DNA repair in bacteria and mammalian cells.
    Annu Rev Biochem. 1979;48:783-836 PMID: 382997
  4. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  5. Nucleotide sequence determination of a strong promoter of the colicin E 1 plasmid. Analysis of restriction sites protected by RNA polymerase interactions before and after limited transcription.
    Nucleic Acids Res. 1979 Nov 10;7(5):1247-62 PMID: 390498
  6. Identification of the lexA gene product of Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6147-51 PMID: 160562
  7. Organization of the recA gene of Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):313-7 PMID: 6244554
  8. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  9. The lexA gene product represses its own promoter.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1932-6 PMID: 6990417
  10. Sequences of the recA gene and protein.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2611-5 PMID: 6930655
  11. DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2819-23 PMID: 6771759
  12. Transcription of bacteriocinogenic plasmid CloDF13 in vivo and in vitro: structure of the cloacin immunity operon.
    J Bacteriol. 1980 Nov;144(2):579-91 PMID: 6159346
  13. Structure of a promotor on plasmid pMB9 derived from plasmid pSC101.
    Nucleic Acids Res. 1980 Apr 11;8(7):1535-50 PMID: 6253940
  14. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd.
    Gene. 1980 Oct;11(1-2):129-48 PMID: 6254849
  15. Evidence for the inducibility of the uvrB operon.
    Nature. 1981 Jan 15;289(5794):196-8 PMID: 7005695
  16. Expression of the E. coli uvrA gene is inducible.
    Nature. 1981 Feb 26;289(5800):808-10 PMID: 6780917
  17. Adenosine dimethylation of 16S ribosomal RNA: effect of the methylgroups on local conformational stability as deduced from electrophoretic mobility of RNA fragments in denaturing polyacrylamide gels.
    Nucleic Acids Res. 1981 Jan 24;9(2):267-75 PMID: 7010312
  18. Relief of polarity caused by transposon Tn5: application in mapping a cloned region of the Escherichia coli uvrB locus essential for UV resistance.
    Gene. 1980 Dec;12(1-2):51-61 PMID: 6260590
  19. Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA.
    Genetics. 1966 Jun;53(6):1119-36 PMID: 5335128
  20. Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.
    Eur J Biochem. 1973 Feb 15;33(1):192-200 PMID: 4691350
  21. Angular alteration of the DNA helix by E. coli RNA polymerase.
    Nat New Biol. 1972 Oct 11;239(93):167-70 PMID: 4573694
  22. Determinant of cistron specificity in bacterial ribosomes.
    Nature. 1975 Mar 6;254(5495):34-8 PMID: 803646
  23. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  24. Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):784-8 PMID: 1093168
  25. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.
    Biochemistry. 1975 Oct 21;14(21):4634-8 PMID: 1101952
  26. Rapid isolation of highly active RNA polymerase from Escherichia coli and its subunits by matrix-bound heparin.
    Eur J Biochem. 1975 Dec 1;60(1):51-5 PMID: 1107037
  27. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.
    Cell. 1976 Sep;9(1):91-9 PMID: 788919
  28. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.
    Bacteriol Rev. 1976 Dec;40(4):869-907 PMID: 795416
  29. A mutant of Escherichia coli showing constitutive expression of the lysogenic induction and error-prone DNA repair pathways.
    Proc Natl Acad Sci U S A. 1977 Jan;74(1):300-4 PMID: 319458
  30. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  31. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  32. Dual control for transcription of the galactose operon by cyclic AMP and its receptor protein at two interspersed promoters.
    Cell. 1977 Nov;12(3):847-54 PMID: 200371
  33. Expression of the uvrB gene of Escherichia coli: in vitro construction of a pMB9 uvrB plasmid.
    J Bacteriol. 1978 Feb;133(2):884-96 PMID: 342513
  34. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  35. Further procedures for sequence analysis by computer.
    Nucleic Acids Res. 1978 Mar;5(3):1013-6 PMID: 643613
  36. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  37. Reconstitution of an Escherichia coli repair endonuclease activity from the separated uvrA+ and uvrB+/uvrC+ gene products.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2569-73 PMID: 351611
  38. A computer aided oligonucleotide analysis provides a model sequence for RNA polymerase-promoter recognition in E.coli.
    Nucleic Acids Res. 1978 Oct;5(10):3759-73 PMID: 364417
  39. Simple method for identification of plasmid-coded proteins.
    J Bacteriol. 1979 Jan;137(1):692-3 PMID: 368040
  40. The nucleotide sequence of the transforming HpaI-E fragment of adenovirus type 5 DNA.
    Gene. 1978 Dec;4(4):309-28 PMID: 744489
  41. DNA sequences of promoter regions for rRNA operons rrnE and rrnA in E. coli.
    Cell. 1979 May;17(1):201-9 PMID: 378405
  42. Tandem promoters direct E. coli ribosomal RNA synthesis.
    Cell. 1979 May;17(1):225-34 PMID: 110460
  43. Organization of the lexA gene of Escherichia coli and nucleotide sequence of the regulatory region.
    Nucleic Acids Res. 1981 Feb 11;9(3):529-43 PMID: 6261224
  44. Acetylornithinase of Escherichia coli: partial purification and some properties.
    J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
  45. Expression of the cloned uvrB gene of Escherichia coli: mode of transcription and orientation.
    J Bacteriol. 1979 Jul;139(1):54-63 PMID: 378961
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.21.5623
Published
1981-11-11
Pages
5623-43
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327548
Subset
IM
Databases
GENBANK
J01722, J01723
External Links
PubMed source DOI: 10.1093/nar/9.21.5623 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2