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PMID: 6261224 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Organization of the lexA gene of Escherichia coli and nucleotide sequence of the regulatory region.

Nucleic acids research ·Vol. 9 ·No. 3 ·1981-02-11 ·Pages 529-43

Miki T, Ebina Y, Kishi F, Nakazawa A

Abstract

The product of the lexA gene of Escherichia coli has been shown to regulate expression of the several cellular functions (SOS functions) induced by treatments which abruptly inhibit DNA synthesis. We have cloned and mapped the lexA gene on a small segment of approximately 600 base pairs. The lexA promotor was located by transcription R-loop analysis, and the lexA product of 22,000 daltons was identified by protein synthesis in vitro. An unknown gene was found which directed the synthesis of a protein of 35,000 daltons in a region downstream from the lexA gene. Nucleotide sequence of the regulatory region of the lexA gene was determined. The sequence contained inverted repeats homologous to that of the recA regulatory region. These inverted repeats may be recognized by the lexA protein, because the protein is considered to repress both the genes as a common repressor.

MeSH Terms
Bacterial Proteins/biosynthesis,genetics Base Sequence Chromosome Mapping DNA Restriction Enzymes Escherichia coli/genetics Genes Operon Plasmids Repressor Proteins/genetics Transcription, Genetic
Chemicals
Bacterial Proteins Repressor Proteins DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Miki T
Ebina Y
Kishi F
Nakazawa A
References (37)
37 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.3.529
Published
1981-02-11
Pages
529-43
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327220
Subset
IM
Databases
GENBANK
J01643, V00299, V00300
External Links
PubMed source DOI: 10.1093/nar/9.3.529 PMC Full Text View journal details
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