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PMID: 2212944 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vivo induction of anergy in peripheral V beta 8+ T cells by staphylococcal enterotoxin B.

The Journal of experimental medicine ·Vol. 172 ·No. 4 ·1990-10-01 ·Pages 1091-100

Rellahan BL, Jones LA, Kruisbeek AM, Fry AM, Matis LA

Abstract

We have developed a model of peripheral in vivo T cell tolerance that is induced by administration of the protein superantigen staphylococcal enterotoxin B (SEB). Rather than activating V beta 8+ T cells, in vivo administration of SEB induced in them a profound state of anergy. This was shown by their failure to proliferate to subsequent in vitro restimulation with SEB or to anti-V beta 8 antibodies. This unresponsiveness was V beta 8 specific since T cells from SEB-immunized mice responded normally to other antigens. 8 d after SEB administration, there was no reduction in the number of V beta 8+ T cells or in the intensity of V beta 8 T cell receptor (TCR) expression. Although a portion of the V beta 8+ T cells from SEB-primed mice were able to express interleukin 2 receptors (IL-2Rs), they failed to proliferate in response to exogenous IL-2, indicating they were defective in their IL-2 responsiveness. 2-4 wk after SEB administration, there was a reduction of approximately 50% in the number of V beta 8+ cells in immunized compared with control animals. There was, however, no reduction in the level of TCR expression on the remaining V beta 8+ cells. These data demonstrate that proteins that activate T cells in vitro in a V beta-specific manner can induce a state of anergy in peripheral T cells in vivo and may possibly further mediate clonal deletion in a portion of the tolerized cells.

MeSH Terms
Animals Enterotoxins/immunology,pharmacology Immune Tolerance Immunization Interleukin-2/pharmacology Lymphocyte Activation Mice Mice, Inbred BALB C Receptors, Antigen, T-Cell/analysis Receptors, Interleukin-2/analysis Staphylococcus aureus T-Lymphocytes/drug effects,immunology
Chemicals
Enterotoxins Interleukin-2 Receptors, Antigen, T-Cell Receptors, Interleukin-2 enterotoxin B, staphylococcal
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rellahan B L
Division of Cytokine Biology, Food and Drug Administration, National Institutes of Health, Bethesda, Maryland 20892.
Jones L A
Kruisbeek A M
Fry A M
Matis L A
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.172.4.1091
Published
1990-10-01
Pages
1091-100
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188615
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.172.4.1091 PMC Full Text View journal details
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