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PMID: 22138992 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cultivated single-stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA-binding stains.

Applied and environmental microbiology ·Vol. 78 ·No. 3 ·2012-02-00 ·Pages 892-4

Holmfeldt K, Odić D, Sullivan MB, Middelboe M, Riemann L

Abstract

This is the first description of cultivated icosahedral single-stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA-binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.

MeSH Terms

Bacteriophages/genetics,isolation & purification Bacteroidetes/virology DNA, Single-Stranded/genetics,isolation & purification Microscopy, Electron Seawater/virology Staining and Labeling/methods Viral Load Virion/ultrastructure Virology/methods

Chemicals

DNA, Single-Stranded

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Holmfeldt Karin

Department of Natural Sciences, Linneaus University, Kalmar, Sweden. [email protected]

Odić Duško

Sullivan Matthew B

Middelboe Mathias

Riemann Lasse

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14 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

1098-5336

DOI

10.1128/AEM.06580-11

[email protected]

Published

2012-02-00

Epub

2011-00-02

Pages

892-4

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC3264134

Subset

External Links

PubMed source DOI: 10.1128/AEM.06580-11 PMC Full Text View journal details

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Cultivated single-stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA-binding stains.

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