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PMID: 2215213 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Review

Recent advances in peptide chain termination.

Molecular microbiology ·Vol. 4 ·No. 6 ·1990-06-00 ·Pages 861-5

Craigen WJ, Lee CC, Caskey CT

Abstract

Peptide chain termination occurs when a stop codon is decoded by a release factor. In Escherichia coli two codon-specific release factors (RF1 and RF2) direct the termination of protein synthesis, while in eukaryotes a single factor is required. The E. coli factors have been purified and their genes isolated. A combination of protein and DNA sequence data reveal that the RFs are structurally similar and that RF2 is encoded in two reading frames. Frame-shifting from one reading frame to the next occurs at a rate of 50%, is regulated by the RF2-specific stop codon UGA, and involves the direct interaction of the RF2 mRNA with the 3' end of the 16S rRNA. The RF genes are located in two separate operons, with the RF1 gene located at 26.7 min and the RF2 gene at 62.3 min on the chromosome map. Ribosomal binding studies place the RF-binding region at the interface between the ribosomal subunits. A possible mechanism of stop-codon recognition is reviewed.

MeSH Terms
Codon Escherichia coli/genetics Peptide Chain Termination, Translational Peptide Termination Factors/biosynthesis,genetics Ribosomes/metabolism
Chemicals
Codon Peptide Termination Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Craigen W J
Institute for Molecular Genetics, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030.
Lee C C
Caskey C T
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47 references, click to expand
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Article Info
Journal
Molecular microbiology
Abbr.
Mol Microbiol
ISSN
0950-382X
DOI
10.1111/j.1365-2958.1990.tb00658.x
Published
1990-06-00
Pages
861-5
Language
English
Region
England
NLM ID
8712028
PMCID
PMC7168415
Subset
IM
Grants
NIGMS NIH HHS · GM34438 · United States
External Links
PubMed source DOI: 10.1111/j.1365-2958.1990.tb00658.x PMC Full Text View journal details
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