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PMID: 2560134 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of herC, a mutation of Escherichia coli affecting maintenance of ColE1.

Molecular & general genetics : MGG ·Vol. 219 ·No. 3 ·1989-11-00 ·Pages 333-40

Kawakami K, Naito S, Inoue N, Nakamura Y, Ikeda H, Uchida H

Abstract

Two modes of ColE1 DNA replication are known, one dependent on RNase H, and the other RNase H independent. The cer114 mutant of the ColE1 replicon is defective in both modes and carries a single base pair alteration 95 bp upstream of the replication origin. An Escherichia coli mutant which restored maintenance of the cer114 replicon was isolated. This host suppressor mutant is defective in RNase H and carries a herC mutation located at 62 min of the E. coli chromosome. The herC mutation is recessive to its wild-type allele and supports maintenance of the mutant replicon in the absence of RNase H. The herC mutation alone conferred cold-sensitive growth, suggesting that the herC gene product is essential for cell growth. The 1832 bp E. coli DNA fragment, containing the wild-type allele of the herC mutation, was cloned and an open reading frame for the HerC protein was determined.

MeSH Terms
Amino Acid Sequence Bacteriocin Plasmids/genetics Base Sequence Cloning, Molecular DNA Replication DNA Transposable Elements Endoribonucleases Escherichia coli/genetics Molecular Sequence Data Plasmids/genetics Ribonuclease H Suppression, Genetic
Chemicals
DNA Transposable Elements Endoribonucleases Ribonuclease H
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kawakami K
Institute of Medical Science, University of Tokyo, Japan.
Naito S
Inoue N
Nakamura Y
Ikeda H
Uchida H
References (34)
34 references, click to expand
  1. Studies on radiation-sensitive mutants of E. coli. 3. Participation of the rec system in induction of mutation by ultraviolet irradiation.
    Mol Gen Genet. 1968;103(1):1-10 PMID: 4890247
  2. Origin of replication of colicin E1 plasmid DNA.
    Proc Natl Acad Sci U S A. 1977 May;74(5):1865-9 PMID: 325558
  3. RNase H and replication of ColE1 DNA in Escherichia coli.
    J Bacteriol. 1986 Apr;166(1):143-7 PMID: 2870052
  4. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  5. The steric effect in lysogenization by bacteriophage lambda. I. Lysogenization of a partially diploid strain of Escherichia coli K-12.
    Virology. 1965 Nov;27(3):329-39 PMID: 5321952
  6. Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda.
    Genetics. 1978 Nov;90(3):427-61 PMID: 365678
  7. IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI.
    J Bacteriol. 1965 Aug;90:554-5 PMID: 14329476
  8. Positive selection for loss of tetracycline resistance.
    J Bacteriol. 1980 Aug;143(2):926-33 PMID: 6259126
  9. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.
    Gene. 1983 Nov;25(2-3):167-78 PMID: 6363212
  10. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12.
    J Bacteriol. 1973 Feb;113(2):798-812 PMID: 4570607
  11. [System of repression insuring immunity in lysogenic bacteria].
    C R Hebd Seances Acad Sci. 1959 Jun 1;248(22):3219-21 PMID: 13663329
  12. Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12.
    Genetics. 1954 Jul;39(4):440-52 PMID: 17247495
  13. Control of primer formation for ColE1 plasmid replication: conformational change of the primer transcript.
    Cell. 1986 Jan 17;44(1):125-36 PMID: 2416472
  14. Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2450-4 PMID: 6156450
  15. Chromosomal location and structure of the operon encoding peptide-chain-release factor 2 of Escherichia coli.
    Proc Natl Acad Sci U S A. 1988 Aug;85(15):5620-4 PMID: 2456575
  16. Absence of RNase H allows replication of pBR322 in Escherichia coli mutants lacking DNA polymerase I.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7845-9 PMID: 6096862
  17. A new conditional lethal mutator (dnaQ49) in Escherichia coli K12.
    Mol Gen Genet. 1978 Jul 25;163(3):277-83 PMID: 355854
  18. Transducing fragments in generalized transduction by phage P1. I. Molecular origin of the fragments.
    J Mol Biol. 1965 Nov;14(1):85-109 PMID: 5883923
  19. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  20. Escherichia coli K-12 F-prime factors, old and new.
    Bacteriol Rev. 1972 Dec;36(4):587-607 PMID: 4568765
  21. Isolation and characterization of a ribonuclease 3 deficient mutant of Escherichia coli.
    Mol Gen Genet. 1973 Oct 16;126(1):53-9 PMID: 4591369
  22. Identification of transfer RNA suppressors in Escherichia coli. I. Amber suppressor su+2, an anticodon mutant of tRNA2Gln.
    J Mol Biol. 1979 Aug 25;132(4):649-62 PMID: 160949
  23. Effects of rifampicin, streptolydigin and actinomycin D on the replication of Col E1 plasmid DNA in Escherichia coli.
    J Mol Biol. 1973 Apr 15;75(3):503-13 PMID: 4579715
  24. Identification of the dnaQ gene product and location of the structural gene for RNase H of Escherichia coli by cloning of the genes.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3770-4 PMID: 6267604
  25. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1.
    Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538-44 PMID: 4922634
  26. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.
    Cell. 1987 Jul 31;50(3):495-508 PMID: 3038334
  27. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  28. Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments.
    Nature. 1974 Oct 11;251(5475):476-81 PMID: 4608939
  29. Escherichia coli mutants suppressing replication-defective mutations of the ColE1 plasmid.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):550-4 PMID: 6320195
  30. Conditionally lethal and recessive UGA-suppressor mutations in the prfB gene encoding peptide chain release factor 2 of Escherichia coli.
    J Bacteriol. 1988 Nov;170(11):5378-81 PMID: 3053663
  31. The codon preference plot: graphic analysis of protein coding sequences and prediction of gene expression.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):539-49 PMID: 6694906
  32. Multiple mechanisms for initiation of ColE1 DNA replication: DNA synthesis in the presence and absence of ribonuclease H.
    Cell. 1987 Dec 24;51(6):1113-22 PMID: 2446774
  33. Initiation of DNA replication in a ColE1-type plasmid: isolation of mutations in the ori region.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6744-8 PMID: 6256758
  34. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00259604
Published
1989-11-00
Pages
333-40
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00259604
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