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PMID: 2217200 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The gamma subunit of transducin is farnesylated.

Lai RK, Perez-Sala D, Cañada FJ, Rando RR

Abstract

Protein prenylation with farnesyl or geranylgeranyl moieties is an important posttranslational modification that affects the activity of such diverse proteins as the nuclear lamins, the yeast mating factor mata, and the ras oncogene products. In this article, we show that whole retinal cultures incorporate radioactive mevalonic acid into proteins of 23-26 kDa and one of 8 kDa. The former proteins are probably the "small" guanine nucleotide-binding regulatory proteins (G proteins) and the 8-kDa protein is the gamma subunit of the well-studied retinal heterotrimeric G protein (transducin). After deprenylating purified transducin and its subunits with Raney nickel or methyl iodide/base, the adducted prenyl group can be identified as an all-trans-farnesyl moiety covalently linked to a cysteine residue. Thus far, prenylation reactions have been found to occur at cysteine in a carboxyl-terminal consensus CAAX sequence, where C is the cysteine, A is an aliphatic amino acid, and X is undefined. Both the alpha and gamma subunits of transducin have this consensus sequence, but only the gamma subunit is prenylated. Therefore, the CAAX motif is not necessary and sufficient to direct prenylation. Finally, since transducin is the best understood G protein, both structurally and mechanistically, the discovery that it is farnesylated should allow for a quantitative understanding of this post-translational modification.

MeSH Terms
Animals Cattle Diterpenes/analysis Farnesol/analysis Gas Chromatography-Mass Spectrometry In Vitro Techniques Leucine/metabolism Macromolecular Substances Mevalonic Acid/metabolism Protein Processing, Post-Translational Retina/metabolism Transducin/biosynthesis,chemistry,genetics
Chemicals
Diterpenes Macromolecular Substances Farnesol geranylgeraniol Transducin Leucine Mevalonic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lai R K
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115.
Perez-Sala D
Cañada F J
Rando R R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1990-10-00
Pages
7673-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54810
Subset
IM
Grants
NEI NIH HHS · EY 03624 · United States
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