Home LiteratureArticle Details
PMID: 2226492 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Use of a ribosomal RNA gene probe for the epidemiological study of methicillin and ciprofloxacin resistant Staphylococcus aureus.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology ·Vol. 9 ·No. 9 ·1990-09-00 ·Pages 649-53

Hadorn K, Lenz W, Kayser FH, Shalit I, Krasemann C

Abstract

Conventional bacteriophage typing was combined with ribotyping in the analysis of methicillin and ciprofloxacin resistant Staphylococcus aureus strains isolated in increasing frequency since the introduction of the new 4-quinolones as therapeutic agents in the Tel-Aviv Medical Center. Whole-cell DNA was digested with EcoRI and HindIII restriction endonucleases. Agarose gel electrophoresis, Southern blotting, and hybridization by biotinylated probe DNA coding for ribosomal RNA revealed 7 to 14 bands. Analysis of the patterns established a single DNA type in EcoRI as well as in HindIII digests for all strains except one. Control strains from other sources differed in their band patterns. Bacteriophage typing confirmed the results of DNA typing. Thus, the frequent occurrence of staphylococcal isolates resistant to 4-quinolones in the hospital was not due to mutational development of resistance in many strains, but to the spread of a resistant strain.

MeSH Terms
Anti-Infective Agents/therapeutic use Bacteriophage Typing Ciprofloxacin/pharmacology Drug Resistance, Microbial Methicillin Resistance/genetics RNA Probes/genetics RNA, Ribosomal/genetics Staphylococcus aureus/drug effects,genetics,isolation & purification
Chemicals
Anti-Infective Agents RNA Probes RNA, Ribosomal Ciprofloxacin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hadorn K
Institute of Medical Microbiology, University of Zürich, Switzerland.
Lenz W
Kayser F H
Shalit I
Krasemann C
References (17)
17 references, click to expand
  1. Widespread quinolone resistance among methicillin-resistant Staphylococcus aureus isolates in a general hospital.
    Antimicrob Agents Chemother. 1989 Apr;33(4):593-4 PMID: 2729953
  2. Ribosomal RNA gene restriction patterns provide increased sensitivity for typing Salmonella typhi strains.
    J Infect Dis. 1989 Jul;160(1):145-9 PMID: 2471748
  3. Cloned, random chromosomal sequences as probes to identify Salmonella species.
    J Infect Dis. 1986 Jul;154(1):156-62 PMID: 3011919
  4. Characterization of Staphylococcus species by ribosomal RNA gene restriction patterns.
    J Gen Microbiol. 1989 Apr;135(4):989-99 PMID: 2480992
  5. Plasmid analysis in molecular epidemiology: a summary and future directions.
    Rev Infect Dis. 1986 Sep-Oct;8(5):738-46 PMID: 3491406
  6. A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA.
    J Infect Dis. 1988 Feb;157(2):280-6 PMID: 2447202
  7. Strategies for typing and properties of epidemic methicillin-resistant Staphylococcus aureus.
    Eur J Clin Microbiol. 1986 Dec;5(6):702-9 PMID: 3643106
  8. Characterization and use of a DNA probe as an epidemiological marker for Pseudomonas aeruginosa.
    J Infect Dis. 1987 Jan;155(1):119-26 PMID: 3098862
  9. Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli.
    Plasmid. 1981 Jul;6(1):112-8 PMID: 7025054
  10. Experimental and clinical aspects of resistance determinants. Mode of resistance against beta-lactam antibiotics in staphylococci.
    Ann N Y Acad Sci. 1971 Jun 11;182:106-17 PMID: 5285286
  11. Molecular typing of methicillin and gentamicin resistant Staphylococcus aureus in Dublin.
    Eur J Clin Microbiol. 1986 Dec;5(6):719-25 PMID: 3026803
  12. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools.
    Ann Inst Pasteur Microbiol. 1986 Sep-Oct;137B(2):165-75 PMID: 2446639
  13. Diagnostic deoxyribonucleic acid probes for infectious diseases.
    Clin Microbiol Rev. 1988 Jan;1(1):82-101 PMID: 3060247
  14. Studies on the molecular epidemiology of diphtheria.
    Lancet. 1983 Oct 22;2(8356):923-6 PMID: 6138500
  15. Studies on the selectivity of DNA precipitation by spermine.
    Nucleic Acids Res. 1981 Oct 24;9(20):5493-504 PMID: 7029471
  16. Characterization of incompatibility group HI1 plasmids from Salmonella typhi by restriction endonuclease digestion and hybridization of DNA probes for Tn3, Tn9, and Tn10.
    Can J Microbiol. 1985 Aug;31(8):721-9 PMID: 3000567
  17. Methicillin-resistant staphylococci 1965-75.
    Lancet. 1975 Oct 4;2(7936):650-3 PMID: 52016
Article Info
Journal
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
Abbr.
Eur J Clin Microbiol Infect Dis
ISSN
0934-9723
DOI
10.1007/BF01964265
Published
1990-09-00
Pages
649-53
Language
English
Region
Germany
NLM ID
8804297
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01964265 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2