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PMID: 22291972 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The progeny of Arabidopsis thaliana plants exposed to salt exhibit changes in DNA methylation, histone modifications and gene expression.

PloS one ·Vol. 7 ·No. 1 ·2012-00-00 ·Pages e30515

Bilichak A, Ilnystkyy Y, Hollunder J, Kovalchuk I

Abstract

Plants are able to acclimate to new growth conditions on a relatively short time-scale. Recently, we showed that the progeny of plants exposed to various abiotic stresses exhibited changes in genome stability, methylation patterns and stress tolerance. Here, we performed a more detailed analysis of methylation patterns in the progeny of Arabidopsis thaliana (Arabidopsis) plants exposed to 25 and 75 mM sodium chloride. We found that the majority of gene promoters exhibiting changes in methylation were hypermethylated, and this group was overrepresented by regulators of the chromatin structure. The analysis of DNA methylation at gene bodies showed that hypermethylation in the progeny of stressed plants was primarily due to changes in the 5' and 3' ends as well as in exons rather than introns. All but one hypermethylated gene tested had lower gene expression. The analysis of histone modifications in the promoters and coding sequences showed that hypermethylation and lower gene expression correlated with the enrichment of H3K9me2 and depletion of H3K9ac histones. Thus, our work demonstrated a high degree of correlation between changes in DNA methylation, histone modifications and gene expression in the progeny of salt-stressed plants.

MeSH Terms
Arabidopsis/drug effects,genetics,metabolism,physiology Breeding Cluster Analysis DNA Methylation/drug effects,genetics,physiology Dose-Response Relationship, Drug Gene Expression Regulation, Plant/drug effects,genetics Genes, Reporter/drug effects Histones/metabolism Plants, Genetically Modified Promoter Regions, Genetic/drug effects Protein Processing, Post-Translational/drug effects,genetics Reproduction, Asexual/physiology Salt Tolerance/drug effects,genetics Salt-Tolerant Plants/drug effects,genetics,metabolism Sodium Chloride/pharmacology Stress, Physiological/genetics,physiology
Chemicals
Histones Sodium Chloride
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bilichak Andriy
Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta, Canada.
Ilnystkyy Yaroslav
Hollunder Jens
Kovalchuk Igor
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0030515
Published
2012-00-00
Epub
2012-00-23
Pages
e30515
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3264603
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0030515 PMC Full Text View journal details
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