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PMID: 2244872 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular cloning of the mammalian fatty acid synthase gene and identification of the promoter region.

The Biochemical journal ·Vol. 271 ·No. 3 ·1990-11-01 ·Pages 675-9

Amy CM, Williams-Ahlf B, Naggert J, Smith S

Abstract

Rat genomic clones encompassing the entire fatty acid synthase gene have been isolated and characterized. The gene is present in a single copy of approx. 20 kb. Genomic DNA sequencing, direct RNA sequencing and S1 nuclease analysis showed that transcription is initiated primarily 1274 nucleotides upstream from the translation start site and that the 87-nucleotide-long 5'-untranslated mRNA sequence is the same in liver, lung and mammary gland. The 5'-flanking region and first intron contain several sequence elements which may be involved in the transcriptional regulation of this gene.

MeSH Terms

Animals Base Sequence Blotting, Southern Cloning, Molecular DNA/genetics Fatty Acid Synthases/genetics Genomic Library Molecular Sequence Data Promoter Regions, Genetic/genetics Rats Rats, Inbred Strains Transcription, Genetic/genetics

Chemicals

DNA Fatty Acid Synthases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Amy C M

Children's Hospital Oakland Research Institute, CA 94609.

Williams-Ahlf B

Naggert J

Smith S

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26 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2710675

Published

1990-11-01

Pages

675-9

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1149615

Subset

Grants

NIDDK NIH HHS · DK 16073 · United States

Databases

GENBANK

X54671

External Links

PubMed source DOI: 10.1042/bj2710675 PMC Full Text View journal details

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Molecular cloning of the mammalian fatty acid synthase gene and identification of the promoter region.

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