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PMID: 2245473 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and properties of yeast mutants affected in farnesyl diphosphate synthetase.

Current genetics ·Vol. 18 ·No. 1 ·1990-07-00 ·Pages 41-6

Chambon C, Ladeveze V, Oulmouden A, Servouse M, Karst F

Abstract

Two yeast mutant strains auxotrophic for ergosterol and blocked in farnesyl diphosphate synthetase (EC 2.5.1.1) were isolated. Genetic analysis has shown that these mutant strains carry additional mutations in the ergosterol pathway besides erg20-1 and erg20-2 which affect FPP synthetase. The novel feature of these mutants is their ability to excrete prenyl alcohols (farnesol and geraniol). As geraniol is toxic for yeast cells, the above leaky mutations in FPP synthetase have to be associated with others in the sterol pathway, in order to slow down geraniol synthesis.

MeSH Terms

Acyclic Monoterpenes Cell Membrane Permeability Dimethylallyltranstransferase/genetics,metabolism Ergosterol/genetics,metabolism Farnesol/metabolism Genes, Fungal Genetic Complementation Test Mevalonic Acid/metabolism Mutation Phenotype Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics,isolation & purification Terpenes/metabolism

Chemicals

Acyclic Monoterpenes Terpenes Farnesol Dimethylallyltranstransferase geraniol Mevalonic Acid Ergosterol

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Chambon C

Laboratoire de Biochimie et Génétique des Microorganismes, Université de Poitiers, France.

Ladeveze V

Oulmouden A

Servouse M

Karst F

References (14)

14 references, click to expand

Cloning, sequencing and analysis of the yeast S. uvarum ERG10 gene encoding acetoacetyl CoA thiolase.
Curr Genet. 1988 Jun;13(6):471-8 PMID: 2900076
Regulation of early enzymes of ergosterol biosynthesis in Saccharomyces cerevisiae.
Biochem J. 1986 Dec 1;240(2):541-7 PMID: 2880580
Geraniol interferes with membrane functions in strains of Candida and Saccharomyces.
Lipids. 1988 Jun;23(6):534-8 PMID: 3050345
Isolation of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase.
Gene. 1990 Apr 16;88(2):253-7 PMID: 2161381
Isolation and characterization of yeast mutants blocked in mevalonic acid formation.
Biochem Biophys Res Commun. 1984 Sep 17;123(2):424-30 PMID: 6148937
Geranyl pyrophosphate synthase: characterization of the enzyme and evidence that this chain-length specific prenyltransferase is associated with monoterpene biosynthesis in sage (Salvia officinalis).
Arch Biochem Biophys. 1989 Jun;271(2):524-35 PMID: 2730002
Substrate Binding of avian liver prenyltransferase.
Biochemistry. 1976 Aug 24;15(17):3739-45 PMID: 182217
Prenyltransferase: the mechanism of the reaction.
Biochemistry. 1976 Mar 9;15(5):1079-83 PMID: 175829
Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway.
Mol Gen Genet. 1977 Sep 9;154(3):269-77 PMID: 200835
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
Gene. 1984 Jun;28(3):351-9 PMID: 6235151
Transformation of yeast.
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
Farnesyl diphosphate synthetase. Molecular cloning, sequence, and expression of an essential gene from Saccharomyces cerevisiae.
J Biol Chem. 1989 Nov 15;264(32):19176-84 PMID: 2681213
Regulation of squalene synthetase and squalene epoxidase activities in Saccharomyces cerevisiae.
Lipids. 1989 Dec;24(12 ):1020-3 PMID: 2693869
Prenyltransferase. Product binding and reaction termination.
J Biol Chem. 1979 Sep 10;254(17):8511-5 PMID: 224056

Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00321113

Published

1990-07-00

Pages

41-6

Language

English

Region

United States

NLM ID

8004904

Subset

External Links

PubMed source DOI: 10.1007/BF00321113 View journal details

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Isolation and properties of yeast mutants affected in farnesyl diphosphate synthetase.

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