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PMID: 2254287 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA sequences required for the alkalophily of Bacillus sp. strain C-125 are located close together on its chromosomal DNA.

Journal of bacteriology ·Vol. 172 ·No. 12 ·1990-12-00 ·Pages 7282-3

Kudo T, Hino M, Kitada M, Horikoshi K

Abstract

Two alkali-sensitive mutants of alkalophilic Bacillus sp. strain C-125 were obtained. Mutant 38154 showed defective regulation of internal pH. Plasmids pALK1 and pALK2, containing DNA fragments different from those of the parent strain, were able to recover alkalophily in mutants 18224 and 38154, respectively. DNA analysis suggested that the two fragments overlapped on the chromosomal DNA of strain C-125.

MeSH Terms
Bacillus/genetics Chromosome Mapping Chromosomes, Bacterial/ultrastructure Cloning, Molecular DNA, Bacterial/genetics Genes, Bacterial Genetic Linkage Hydrogen-Ion Concentration Restriction Mapping
Chemicals
DNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kudo T
Riken Institute, Saitama-ken, Japan.
Hino M
Kitada M
Horikoshi K
References (9)
9 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.12.7282-7283.1990
Published
1990-12-00
Pages
7282-3
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210858
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.12.7282-7283.1990 PMC Full Text View journal details
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