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PMID: 2258706 Published · ppublish English Journal Article

Immunoregulatory role of transforming growth factor beta (TGF-beta) in development of killer cells: comparison of active and latent TGF-beta 1.

The Journal of experimental medicine ·Vol. 172 ·No. 6 ·1990-12-01 ·Pages 1777-84

Wallick SC, Figari IS, Morris RE, Levinson AD, Palladino MA

Abstract

Using recombinant DNA technology, we have generated Chinese hamster ovary (CHO) cell lines that synthesize latent transforming growth factor beta 1 (TGF-beta 1) to study immune regulation by TGF-beta 1. In vitro, latent TGF-beta 1 synthesized by transfectants or added exogenously as a purified complex after activation inhibited CTL generation to a similar extent as seen with acid-activated recombinant human (rHu) TGF-beta 1. In vivo, serum from nu/nu mice bearing CHO/TGF-beta 1 tumors contained significant levels of latent TGF-beta 1 in addition to depressed natural killer (NK) activity in spleens which paralleled that seen in C3H/HeJ mice treated with acid-activated rHuTGF-beta 1. rHuTGF-beta 1 treatment of mice receiving heart allografts resulted in significant enhancement of organ graft survival. Because of possible regulated tissue-specific activation, administration of latent rather than active TGF-beta may provide a better route to deliver this powerful immunosuppressive agent in vivo.

MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic Cricetinae Cricetulus Female Graft Survival Heart Transplantation/immunology Humans Killer Cells, Natural/drug effects,immunology Mice Mice, Inbred C57BL Mice, Nude Ovary Recombinant Proteins/pharmacology T-Lymphocytes, Cytotoxic/drug effects,immunology Transfection Transforming Growth Factor beta/genetics,immunology,pharmacology
Chemicals
Recombinant Proteins Transforming Growth Factor beta
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wallick S C
Department of Immunology Research and Assay Technologies Genetech, Inc., South San Francisco, California 94080.
Figari I S
Morris R E
Levinson A D
Palladino M A
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.172.6.1777
Published
1990-12-01
Pages
1777-84
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188774
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.172.6.1777 PMC Full Text View journal details
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