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PMID: 2788703 Published · ppublish English Journal Article

Transforming growth factor beta specifically enhances IgA production by lipopolysaccharide-stimulated murine B lymphocytes.

The Journal of experimental medicine ·Vol. 170 ·No. 3 ·1989-09-01 ·Pages 1039-44

Coffman RL, Lebman DA, Shrader B

Abstract

The addition of TGF-beta to cultures of LPS-stimulated murine B cells causes a approximately 10-fold enhancement of IgA production, yet causes a 10-fold decrease in total Ig production. IL-2 and, to a lesser extent, IL-5 synergize with TGF-beta to further enhance IgA production and partially reverse the inhibition of total Ig production. IgA constitutes only approximately 0.1% of total Ig in LPS-stimulated cultures, but that percentage rises to 15-25% in cultures to which TGF-beta and IL-2 are added. TGF-beta induces a substantial increase in IgA production from sIgA- B cells but inhibits IgA production by sIgA+ cells. This finding suggests that TGF-beta acts as an isotype-specific switch factor for IgA.

MeSH Terms
Animals B-Lymphocytes/immunology Female Immunoglobulin A/biosynthesis Interleukin-2/pharmacology Interleukin-5 Interleukins/pharmacology Lipopolysaccharides/pharmacology Mice Mice, Inbred BALB C Receptors, Antigen, B-Cell/analysis Transforming Growth Factors/pharmacology
Chemicals
Immunoglobulin A Interleukin-2 Interleukin-5 Interleukins Lipopolysaccharides Receptors, Antigen, B-Cell Transforming Growth Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Coffman R L
Department of Immunology, DNAX Research Institute, Palo Alto, California 94304.
Lebman D A
Shrader B
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18 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.170.3.1039
Published
1989-09-01
Pages
1039-44
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189449
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.170.3.1039 PMC Full Text View journal details
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