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PMID: 2263461 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Ultrastructure of transcriptionally competent chromatin.

Nucleic acids research ·Vol. 18 ·No. 23 ·1990-12-11 ·Pages 7015-24

Locklear L, Ridsdale JA, Bazett-Jones DP, Davie JR

Abstract

We have examined a salt-soluble, transcriptionally competent gene-enriched fraction of chicken erythrocyte chromatin and compared it to bulk chromatin using the unique microanalytical capabilities of Electron Spectroscopic Imaging (ESI). The salt-soluble fraction is enriched 48 fold in beta-globin gene sequences and is also enriched in histones that are post-synthetically modified, including acetylation and ubiquitination. Differences between the two fractions are also apparent in the distribution of the two major forms of nucleoprotein structures, including (1) particles which present a circular profile and possess protein and DNA content nearly identical to that of the canonical nucleosome and account for 89% of particles in the bulk fraction but account for only 66% of the particles in the competent fraction, and (2) u-shaped particles which possess about 20% less protein mass than particles of circular profile and are about 10x more prevalent in the transcriptionally competent fraction than in the bulk. Additionally, elongated particles with protein and DNA content similar to the u-shaped objects are also seen in the competent fraction.

MeSH Terms
Acetylation Animals Chickens Chromatin/chemistry,metabolism,ultrastructure DNA/analysis Electron Probe Microanalysis Erythrocytes Genes Globins/genetics Histones/genetics,metabolism Nucleosomes/metabolism Proteins/analysis Salts Solubility Transcription, Genetic
Chemicals
Chromatin Histones Nucleosomes Proteins Salts Globins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Locklear L
Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
Ridsdale J A
Bazett-Jones D P
Davie J R
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32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.23.7015
Published
1990-12-11
Pages
7015-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC332764
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.23.7015 PMC Full Text View journal details
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