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PMID: 22645547 Published · epublish English Journal Article

Molecular screening tools to study Arabidopsis transcription factors.

Frontiers in plant science ·Vol. 2 ·2011-00-00 ·Pages 68

Wehner N, Weiste C, Dröge-Laser W

Abstract

In the model plant Arabidopsis thaliana, more than 2000 genes are estimated to encode transcription factors (TFs), which clearly emphasizes the importance of transcriptional control. Although genomic approaches have generated large TF open reading frame (ORF) collections, only a limited number of these genes is functionally characterized, yet. This review evaluates strategies and methods to identify TF functions. In particular, we focus on two recently developed TF screening platforms, which make use of publically available GATEWAY(®)-compatible ORF collections. (1) The Arabidopsis thalianaTF ORF over-Expression (AtTORF-Ex) library provides pooled collections of transgenic lines over-expressing HA-tagged TF genes, which are suited for screening approaches to define TF functions in stress defense and development. (2) A high-throughput microtiter plate based protoplast transactivation (PTA) system has been established to screen for TFs which are regulating a given promoter:Luciferase construct in planta.

Keywords
Arabidopsis thaliana screening tools transcription factor function
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wehner Nora
Julius-von-Sachs-Institute, University of Würzburg Würzburg, Germany.
Weiste Christoph
Dröge-Laser Wolfgang
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Article Info
Journal
Frontiers in plant science
Abbr.
Front Plant Sci
ISSN
1664-462X
DOI
10.3389/fpls.2011.00068
Published
2011-00-00
Epub
2011-00-04
Pages
68
Language
English
Region
Switzerland
NLM ID
101568200
PMCID
PMC3355788
External Links
PubMed source DOI: 10.3389/fpls.2011.00068 PMC Full Text View journal details
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