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PMID: 2265570 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Meiotic chromosome behaviour reflects levels of sequence divergence in Sus scrofa domestica satellite DNA.

Chromosoma ·Vol. 99 ·No. 5 ·1990-09-00 ·Pages 330-5

Jantsch M, Hamilton B, Mayr B, Schweizer D

Abstract

We present a general model for the evolution of chromosome-specific satellite DNA subfamilies. Sus scrofa domestica has a bimodal karyotype with two autosomal subsets of 12 meta-/submetacentric (Mc) and 6 acrocentric (Ac) chromosome types (Mc and Ac "subgenomes"). We show that the centromeric heterochromatin is characterised by two distinct satellite DNA families designed Mc1 and Ac2. Mc1 is a diverse satellite family of the Mc subgenome of which certain members with a 100 bp repeat unit are found to occur at the pericentromeric regions of each Mc autosome, while others are chromosome-specific, e.g. clone Mc pAv1.5, a higher order repeat variant, which hybridises specifically to chromosome 1. Ac2 is a homogeneous satellite occurring at the subterminal pericentromeric regions of all Ac autosomes. DNA sequence analyses showed that all clones investigated are built up from a 14 bp repeat unit which is highly conserved. In situ hybridisation to meiotic pachytene nuclei revealed a distinct spatial arrangement of the Ac2 centromeric satellite.

MeSH Terms
Animals Base Sequence Biological Evolution Chromosome Banding Consensus Sequence DNA, Satellite/chemistry Genetic Variation Heterochromatin/ultrastructure Karyotyping Meiosis Models, Genetic Molecular Sequence Data Nucleic Acid Conformation Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Swine/genetics Y Chromosome/ultrastructure
Chemicals
DNA, Satellite Heterochromatin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jantsch M
Department of Cytology and Genetics, University of Vienna, Austria.
Hamilton B
Mayr B
Schweizer D
References (18)
18 references, click to expand
  1. Heterochromatin and nucleolus-organizer-region behaviour at male pachytene of Sus scrofa domestica.
    Chromosoma. 1984;91(1):12-9 PMID: 6543170
  2. Colony hybridization.
    Methods Enzymol. 1979;68:379-89 PMID: 542125
  3. Mouse major (gamma) satellite DNA is highly conserved and organized into extremely long tandem arrays: implications for recombination between nonhomologous chromosomes.
    Genomics. 1989 Oct;5(3):407-14 PMID: 2613229
  4. Analysis of highly purified satellite DNA containing chromatin from the mouse.
    Nucleic Acids Res. 1982 Mar 11;10(5):1481-94 PMID: 7071018
  5. Sensitive detection of hybridocytochemical results by means of reflection-contrast microscopy.
    J Histochem Cytochem. 1985 Dec;33(12):1241-6 PMID: 2415575
  6. A male-specific repeated DNA sequence in the domestic pig.
    Nucleic Acids Res. 1988 Nov 11;16(21):10389 PMID: 3194222
  7. Chromosome-specific subsets of human alpha satellite DNA: analysis of sequence divergence within and between chromosomal subsets and evidence for an ancestral pentameric repeat.
    J Mol Evol. 1987;25(3):207-14 PMID: 2822935
  8. Multiple satellite deoxyribonucleic acids in the calf and their relation to the sex chromosomes.
    J Mol Biol. 1973 Dec 15;81(3):273-84 PMID: 4767457
  9. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  10. The constitutive heterochromatin of porcine chromosomes.
    J Hered. 1982 May-Jun;73(3):231-3 PMID: 7096986
  11. The specific organisation of satellite DNA sequences on the X-chromosome of Mus musculus: partial independence of chromosome evolution.
    Nucleic Acids Res. 1980 Feb 25;8(4):781-92 PMID: 6253924
  12. Mammalian repetitive DNA sequences in a stable Robertsonian system. Characterization, in situ hybridizations, and cross-species hybridizations of repetitive DNAs in calf, sheep, and goat chromosomes.
    Cytogenet Cell Genet. 1978;21(3):145-67 PMID: 657846
  13. The distribution of satellite and main-band DNA components in the melanogaster species subgroup of Drosophila. I. Fractionation of DNA in actinomycin D and distamycin A density gradients.
    Chromosoma. 1978 Aug 14;67(4):341-63 PMID: 99293
  14. Homologous subfamilies of human alphoid repetitive DNA on different nucleolus organizing chromosomes.
    Proc Natl Acad Sci U S A. 1987 Feb;84(4):1075-9 PMID: 3469648
  15. Electrophoretic transfer of proteins and nucleic acids from slab gels to diazobenzyloxymethyl cellulose or nitrocellulose sheets.
    Anal Biochem. 1980 Mar 1;102(2):459-71 PMID: 7425302
  16. A general method for isolation of high molecular weight DNA from eukaryotes.
    Nucleic Acids Res. 1976 Sep;3(9):2303-8 PMID: 987581
  17. Structure and properties of a highly repetitive DNA sequence in sheep.
    Nucleic Acids Res. 1984 Mar 12;12(5):2343-50 PMID: 6324115
  18. Multiple sequence alignment with hierarchical clustering.
    Nucleic Acids Res. 1988 Nov 25;16(22):10881-90 PMID: 2849754
Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF01731720
Published
1990-09-00
Pages
330-5
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Databases
GENBANK
X51555, X51558, X51559, X51560
External Links
PubMed source DOI: 10.1007/BF01731720 View journal details
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