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PMID: 22685408 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure-function analysis of barley NLR immune receptor MLA10 reveals its cell compartment specific activity in cell death and disease resistance.

PLoS pathogens ·Vol. 8 ·No. 6 ·2012-00-00 ·Pages e1002752

Bai S, Liu J, Chang C, Zhang L, Maekawa T, Wang Q, Xiao W, Liu Y, Chai J, Takken FL, Schulze-Lefert P, Shen QH

Abstract

Plant intracellular immune receptors comprise a large number of multi-domain proteins resembling animal NOD-like receptors (NLRs). Plant NLRs typically recognize isolate-specific pathogen-derived effectors, encoded by avirulence (AVR) genes, and trigger defense responses often associated with localized host cell death. The barley MLA gene is polymorphic in nature and encodes NLRs of the coiled-coil (CC)-NB-LRR type that each detects a cognate isolate-specific effector of the barley powdery mildew fungus. We report the systematic analyses of MLA10 activity in disease resistance and cell death signaling in barley and Nicotiana benthamiana. MLA10 CC domain-triggered cell death is regulated by highly conserved motifs in the CC and the NB-ARC domains and by the C-terminal LRR of the receptor. Enforced MLA10 subcellular localization, by tagging with a nuclear localization sequence (NLS) or a nuclear export sequence (NES), shows that MLA10 activity in cell death signaling is suppressed in the nucleus but enhanced in the cytoplasm. By contrast, nuclear localized MLA10 is sufficient to mediate disease resistance against powdery mildew fungus. MLA10 retention in the cytoplasm was achieved through attachment of a glucocorticoid receptor hormone-binding domain (GR), by which we reinforced the role of cytoplasmic MLA10 in cell death signaling. Together with our data showing an essential and sufficient nuclear MLA10 activity in disease resistance, this suggests a bifurcation of MLA10-triggered cell death and disease resistance signaling in a compartment-dependent manner.

MeSH Terms
Amino Acid Motifs Cell Death Disease Resistance/physiology Gene Expression Regulation, Plant Hordeum/genetics,immunology,metabolism Mycoses/immunology Plant Diseases/genetics,immunology Plant Proteins/chemistry,genetics,immunology,metabolism Polymerase Chain Reaction Receptors, Cytoplasmic and Nuclear/chemistry,genetics,immunology,metabolism Structure-Activity Relationship
Chemicals
Plant Proteins Receptors, Cytoplasmic and Nuclear
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Bai Shiwei
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Liu Jie
Chang Cheng
Zhang Ling
Maekawa Takaki
Wang Qiuyun
Xiao Wenkai
Liu Yule
Chai Jijie
Takken Frank L W
Schulze-Lefert Paul
Shen Qian-Hua
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Article Info
Journal
PLoS pathogens
Abbr.
PLoS Pathog
ISSN
1553-7374
DOI
10.1371/journal.ppat.1002752
Published
2012-00-00
Epub
2012-00-07
Pages
e1002752
Language
English
Region
United States
NLM ID
101238921
PMCID
PMC3369952
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.ppat.1002752 PMC Full Text View journal details
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