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PMID: 226954 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence heterogeneity at the 5' termini of late simian virus 40 19S and 16S mRNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 7 ·1979-07-00 ·Pages 3078-82

Canaani D, Kahana C, Mukamel A, Groner Y

Abstract

The 5'-cap-containing leader sequence of the most abundant 19S and 16S mRNAs of simian virus 40 (SV40) was previously mapped between 0.67 and 0.76 map units. We now find that the two late mRNA species contain multiple 5' ends. Eight different RNase T2-resistant cap structures were identified:m7GpppmAmpU (47%); m7GpppmAmpUmpU (19%); m7GpppmAmpC (16%); m7GpppmAmpCmpA (5%); m7GpppmAmpG (6%); m7GpppGmpC (3%); m7GpppmAmGmpA (2%); m7GpppGmpCmpG (2%). Capped T1 oligonucleotides of 19S and 16S mRNAs have been isolated by two different procedures: (i) chromatography on a DEAE-cellulose column followed by paper electrophoresis and (ii) two-dimensional electrophoresis/homochromatography. Cap structures of the isolated 5' oligonucleotides were identified. Each of the major caps was found to be associated with a few differential 5' oligonucleotides, implying a vast heterogeneity at the termini of SV40 late mRNAs. The results suggest that on SV40 DNA, RNA polymerase II has a reportoire of initiation points. In most of the cases, initiation takes place with adenosine triphosphate followed by a pyrimidine. Alternatively, transcription may start at one specific point but a unique mechanism of processing generates heterogeneous populations of termini with a common 5' adenosine triphosphate.

MeSH Terms
Base Sequence Methods Oligoribonucleotides/isolation & purification RNA Caps RNA, Messenger/isolation & purification RNA, Viral/isolation & purification Simian virus 40/metabolism
Chemicals
Oligoribonucleotides RNA Caps RNA, Messenger RNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Canaani D
Kahana C
Mukamel A
Groner Y
References (40)
40 references, click to expand
  1. Post-transcriptional and translational controls of gene expression in eukaryotes.
    Annu Rev Biochem. 1978;47:1079-126 PMID: 354491
  2. Methylation and capping of RNA polymerase II primary transcripts by HeLa nuclear homogenates.
    Biochemistry. 1978 Mar 21;17(6):977-82 PMID: 629955
  3. Synthesis methylation, and capping of nuclear RNA by a subcellular system.
    Biochemistry. 1976 Nov 16;15(23):5039-46 PMID: 990262
  4. Rapid sequence determination of late simian virus 40 16S mRNA leader by using inhibitors of reverse transcriptase.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):731-5 PMID: 85304
  5. Gaps and duplicated sequences in the leaders of SV40 16S RNA.
    Nucleic Acids Res. 1978 Nov;5(11):4195-213 PMID: 82936
  6. Multiple 5' terminal cap structures in late polyoma virus RNA.
    Cell. 1979 Feb;16(2):357-71 PMID: 222458
  7. Heterogeneity and 5'-terminal structures of the late RNAs of simian virus 40.
    J Mol Biol. 1978 Dec 25;126(4):813-46 PMID: 218021
  8. Mutational alterations within the simian virus 40 leader segment generate altered 16S and 19S mRNA's.
    J Virol. 1979 Jan;29(1):209-19 PMID: 219218
  9. Mapping the spliced and unspliced late lytic SV40 RNAs.
    Cell. 1978 Aug;14(4):971-82 PMID: 210961
  10. The initiation of transcription of SV40 DNA at late time after infection.
    Virology. 1979 Jan 30;92(2):310-23 PMID: 218346
  11. Enzymatic conversion of 5'-phosphate-terminated RNA to 5'-di- and triphosphate-terminated RNA.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4793-7 PMID: 217000
  12. Sequence arrangement of the 5' ends of simian virus 40 16S and 19S mRNAs.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4982-5 PMID: 200942
  13. Localization of the 5' terminus of late SV40 mRNA.
    Nucleic Acids Res. 1978 Jul;5(7):2359-71 PMID: 209408
  14. Characterization of the 5'-terminal capped structures of late simian virus 40-specific mRNA.
    J Virol. 1978 Mar;25(3):824-30 PMID: 205672
  15. Electron microscopic evidence for splicing of SV40 late mRNAs.
    Cell. 1978 Apr;13(4):783-90 PMID: 207441
  16. Spliced early mRNAs of simian virus 40.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274-8 PMID: 206891
  17. Capping structures of simian virus 40 19S and 16S mRNAs.
    Nucleic Acids Res. 1977 Nov;4(11):3959-68 PMID: 201923
  18. 5'-Terminal sequences and coding region of late simian virus 40 mRNAs are derived from noncontiguous segments of the viral genome.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5323-7 PMID: 202950
  19. The use of nuclease P1 in sequence analysis of end group labeled RNA.
    Nucleic Acids Res. 1977 Dec;4(12):4091-108 PMID: 202925
  20. The 5'-terminal leader sequence of late 16 S mRNA from cells infected with simian virus 40.
    J Biol Chem. 1978 May 25;253(10):3643-7 PMID: 206548
  21. Novel mechanism for RNA maturation: the leader sequences of simian virus 40 mRNA are not transcribed adjacent to the coding sequences.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3686-90 PMID: 198797
  22. Structure of a large segment of the genome of simian virus 40 that does not encode known proteins.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):827-31 PMID: 191835
  23. "Pretranscriptional capping" in the biosynthesis of cytoplasmic polyhedrosis virus mRNA.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1086-90 PMID: 349555
  24. 5'-Terminal capping of RNA by guanylyltransferase from HeLa cell nuclei.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3758-61 PMID: 269428
  25. Spliced segments at the 5' terminus of adenovirus 2 late mRNA.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3171-5 PMID: 269380
  26. Coincidence of the promoter and capped 5' terminus of RNA from the adenovirus 2 major late transcription unit.
    Cell. 1978 Dec;15(4):1463-75 PMID: 729003
  27. The spliced structures of adenovirus 2 fiber message and the other late mRNAs.
    Cell. 1978 Oct;15(2):497-510 PMID: 719751
  28. Quantitative electron microscopy of early adenovirus RNA.
    J Mol Biol. 1977 Nov 5;116(3):525-48 PMID: 592391
  29. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.
    FEBS Lett. 1977 Oct 15;82(2):314-6 PMID: 913604
  30. Two adenovirus mRNAs have a common 5' terminal leader sequence encoded at least 10 kb upstream from their main coding regions.
    Cell. 1977 Sep;12(1):9-21 PMID: 902321
  31. An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA.
    Cell. 1977 Sep;12(1):1-8 PMID: 902310
  32. One predominant 5'-undecanucleotide in adenovirus 2 late messenger RNAs.
    Cell. 1977 Jul;11(3):533-44 PMID: 884734
  33. Capping of eucaryotic mRNAs.
    Cell. 1976 Dec;9(4 PT 2):645-53 PMID: 1017010
  34. 5'-Capping structures of Artemia salina mRNA and the translational inhibition by cap analogs.
    Eur J Biochem. 1976 Dec;71(1):281-93 PMID: 1009952
  35. Isolation and characterization of simian virus 40 ribonucleic acid.
    J Virol. 1972 Aug;10(2):193-201 PMID: 4342237
  36. Biogenesis and characterization of SV40 and polyoma RNAs in productively infected cells.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:165-78 PMID: 169056
  37. Purification of SV-40 messenger RNA by hybridization to SV-40 DNA covalently bound to Sepharose.
    Biochemistry. 1975 Sep 23;14(19):4215-20 PMID: 170957
  38. The role of deoxyribonucleic acid in ribonucleic acid synthesis. XIV. A study of the initiation of ribonucleic acid synthesis.
    J Biol Chem. 1967 Nov 10;242(21):4908-18 PMID: 4862425
  39. Synthesis of RNA containing a methylated blocked 5' terminus by HeLa nuclear homogenates.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):2930-4 PMID: 1059083
  40. The isolation of 3'-terminal polynucleotides from RNA molecules.
    Biochim Biophys Acta. 1971 Aug 26;246(2):337-40 PMID: 5132907
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.7.3078
Published
1979-07-00
Pages
3078-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383766
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.7.3078 PMC Full Text View journal details
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