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PMID: 22707618 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and proteomic characterization of the mouse sperm acrosomal matrix.

Molecular & cellular proteomics : MCP ·Vol. 11 ·No. 9 ·2012-09-00 ·Pages 758-74

Guyonnet B, Zabet-Moghaddam M, SanFrancisco S, Cornwall GA

Abstract

A critical step during fertilization is the sperm acrosome reaction in which the acrosome releases its contents allowing the spermatozoa to penetrate the egg investments. The sperm acrosomal contents are composed of both soluble material and an insoluble material called the acrosomal matrix (AM). The AM is thought to provide a stable structure from which associated proteins are differentially released during fertilization. Because of its important role during fertilization, efforts have been put toward isolating the AM for biochemical study and to date AM have been isolated from hamster, guinea pig, and bull spermatozoa. However, attempts to isolate AM from mouse spermatozoa, the species in which fertilization is well-studied, have been unsuccessful possibly because of the small size of the mouse sperm acrosome and/or its fusiform shape. Herein we describe a procedure for the isolation of the AM from caput and cauda mouse epididymal spermatozoa. We further carried out a proteomic analysis of the isolated AM from both sperm populations and identified 501 new proteins previously not detected by proteomics in mouse spermatozoa. A comparison of the AM proteome from caput and cauda spermatozoa showed that the AM undergoes maturational changes during epididymal transit similar to other sperm domains. Together, our studies suggest the AM to be a dynamic and functional structure carrying out a variety of biological processes as implied by the presence of a diverse group of proteins including proteases, chaperones, hydrolases, transporters, enzyme modulators, transferases, cytoskeletal proteins, and others.

MeSH Terms
Acrosome/chemistry,metabolism Acrosome Reaction Animals Epididymis/cytology Male Mice Proteome/analysis Proteomics Spermatozoa/chemistry,ultrastructure
Chemicals
Proteome
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Guyonnet Benoit
Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Texas Tech University, Lubbock, TX 79430, USA.
Zabet-Moghaddam Masoud
SanFrancisco Susan
Cornwall Gail A
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Article Info
Journal
Molecular & cellular proteomics : MCP
Abbr.
Mol Cell Proteomics
ISSN
1535-9484
DOI
10.1074/mcp.M112.020339
Published
2012-09-00
Epub
2012-00-15
Pages
758-74
Language
English
Region
United States
NLM ID
101125647
PMCID
PMC3434786
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1074/mcp.M112.020339 PMC Full Text View journal details
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