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PMID: 20080865 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Sperm proteomics reveals intensified selection on mouse sperm membrane and acrosome genes.

Molecular biology and evolution ·Vol. 27 ·No. 6 ·2010-06-00 ·Pages 1235-46

Dorus S, Wasbrough ER, Busby J, Wilkin EC, Karr TL

Abstract

Spermatozoa are a focal point for the impact of sexual selection due to sperm competition and sperm-female interactions in a wide range of sexually reproducing organisms. In-depth molecular investigation of the ramifications of these selective regimes has been limited due to a lack of information concerning the molecular composition of sperm. In this study, we utilize three previously published proteomic data sets in conjunction with our whole mouse sperm proteomic analysis to delineate cellular regions of sperm most impacted by positive selection. Interspecific analysis reveals robust evolutionary acceleration of sperm cell membrane genes (which include genes encoding acrosomal and sperm cell surface proteins) relative to other sperm genes, and evidence for positive selection in approximately 22% of sperm cell membrane components was obtained using maximum likelihood models. The selective forces driving the accelerated evolution of these membrane proteins may occur at a number of locations during sperm development, maturation, and transit through the female reproductive tract where the sperm cell membrane and eventually the acrosome are exposed to the extracellular milieu and available for direct cell-cell interactions.

MeSH Terms
Acrosome/metabolism Animals Chromatography, High Pressure Liquid Databases, Protein Evolution, Molecular Male Membrane Proteins/genetics Mice Mice, Inbred BALB C Models, Statistical Organ Specificity Phenotype Proteome/genetics Proteomics/methods Spermatozoa/metabolism,physiology Statistics, Nonparametric Testis/metabolism
Chemicals
Membrane Proteins Proteome
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dorus Steve
Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom.
Wasbrough Elizabeth R
Busby Jennifer
Wilkin Elaine C
Karr Timothy L
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Article Info
Journal
Molecular biology and evolution
Abbr.
Mol Biol Evol
ISSN
1537-1719
DOI
10.1093/molbev/msq007
Published
2010-06-00
Epub
2010-00-15
Pages
1235-46
Language
English
Region
United States
NLM ID
8501455
PMCID
PMC2877994
Subset
IM
Grants
NIGMS NIH HHS · F32 GM076913 · United States
NIGMS NIH HHS · F32GM076913-03 · United States
Biotechnology and Biological Sciences Research Council · United Kingdom
External Links
PubMed source DOI: 10.1093/molbev/msq007 PMC Full Text View journal details
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