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PMID: 2275539 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of lactococci and enterococci by colony hybridization with 23S rRNA-targeted oligonucleotide probes.

Applied and environmental microbiology ·Vol. 56 ·No. 9 ·1990-09-00 ·Pages 2927-9

Betzl D, Ludwig W, Schleifer KH

Abstract

Specific sequences of 23S rRNA of Lactococcus lactis, Enterococcus faecalis, Enteroccus faecium, and Enterococcus malodoratus/Enterococcus avium were identified, and complementary oligonucleotide probes were synthesized. The specificity of the probes was evaluated by dot blot and colony hybridizations. The probes can be used for the specific detection and identification of colonies of the corresponding species in mixed cultures.

MeSH Terms
Base Sequence DNA Probes Leuconostoc/genetics Molecular Sequence Data Nucleic Acid Hybridization RNA, Ribosomal, 23S/genetics Streptococcus/genetics
Chemicals
DNA Probes RNA, Ribosomal, 23S
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Betzl D
Department of Microbiology, Technical University of Munich, Federal Republic of Germany.
Ludwig W
Schleifer K H
References (11)
11 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.56.9.2927-2929.1990
Published
1990-09-00
Pages
2927-9
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC184868
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.56.9.2927-2929.1990 PMC Full Text View journal details
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