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PMID: 228054 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Intracellular forms of simian virus 40 nucleoprotein complexes. II. Biochemical and electron microscopic analysis of simian virus 40 virion assembly.

Journal of virology ·Vol. 31 ·No. 1 ·1979-07-00 ·Pages 199-208

Coca-Prados M, Hsu MT

Abstract

The simian virus 40 virion assembly process was studied with pulse-labeling kinetics of virion proteins, CsCl gradient analysis, electron microscopy, and low-salt gel electrophoresis. The results obtained are consistent with the model of gradual addition and organization of capsid proteins around simian virus 40 chromatin. Empty virions, as observed in the CsCl gradient by previous workers, were found to be the dissociation product of immature virus. Histone H1 was found in simian virus 40 chromatin and virion assembly intermediates but not in the mature virion banding at 1.34 g/ml in the CsCl gradient.

MeSH Terms
Capsid/analysis Centrifugation, Density Gradient DNA, Viral/analysis Electrophoresis, Polyacrylamide Gel Microscopy, Electron Morphogenesis Simian virus 40/ultrastructure Viral Proteins/analysis Virion/ultrastructure
Chemicals
DNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Coca-Prados M
Hsu M T
References (20)
20 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.31.1.199-208.1979
Published
1979-07-00
Pages
199-208
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353436
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.31.1.199-208.1979 PMC Full Text View journal details
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