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PMID: 22822205 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

PROTON GRADIENT REGULATION5 is essential for proper acclimation of Arabidopsis photosystem I to naturally and artificially fluctuating light conditions.

The Plant cell ·Vol. 24 ·No. 7 ·2012-07-00 ·Pages 2934-48

Suorsa M, Järvi S, Grieco M, Nurmi M, Pietrzykowska M, Rantala M, Kangasjärvi S, Paakkarinen V, Tikkanen M, Jansson S, Aro EM

Abstract

In nature, plants are challenged by constantly changing light conditions. To reveal the molecular mechanisms behind acclimation to sometimes drastic and frequent changes in light intensity, we grew Arabidopsis thaliana under fluctuating light conditions, in which the low light periods were repeatedly interrupted with high light peaks. Such conditions had only marginal effect on photosystem II but induced damage to photosystem I (PSI), the damage being most severe during the early developmental stages. We showed that PROTON GRADIENT REGULATION5 (PGR5)-dependent regulation of electron transfer and proton motive force is crucial for protection of PSI against photodamage, which occurred particularly during the high light phases of fluctuating light cycles. Contrary to PGR5, the NAD(P)H dehydrogenase complex, which mediates cyclic electron flow around PSI, did not contribute to acclimation of the photosynthetic apparatus, particularly PSI, to rapidly changing light intensities. Likewise, the Arabidopsis pgr5 mutant exhibited a significantly higher mortality rate compared with the wild type under outdoor field conditions. This shows not only that regulation of PSI under natural growth conditions is crucial but also the importance of PGR5 in PSI protection.

MeSH Terms
Acclimatization/physiology,radiation effects Antioxidants/metabolism Arabidopsis/genetics,physiology,radiation effects Arabidopsis Proteins/genetics,metabolism Cell Respiration/radiation effects Electron Transport/radiation effects Gene Expression Regulation, Plant/radiation effects Light Models, Molecular Mutation Oxidation-Reduction/radiation effects Oxidative Stress/radiation effects Phenotype Photosynthetic Reaction Center Complex Proteins/genetics,metabolism Photosystem I Protein Complex/radiation effects Photosystem II Protein Complex/radiation effects Plant Leaves/genetics,physiology,radiation effects Proton-Motive Force/radiation effects Reactive Oxygen Species/metabolism Seedlings/genetics,physiology,radiation effects
Chemicals
Antioxidants Arabidopsis Proteins PGR5 protein, Arabidopsis Photosynthetic Reaction Center Complex Proteins Photosystem I Protein Complex Photosystem II Protein Complex Reactive Oxygen Species
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Suorsa Marjaana
Department of Biochemistry and Food Chemistry, Molecular Plant Biology, University of Turku, FI-20014 Turku, Finland.
Järvi Sari
Grieco Michele
Nurmi Markus
Pietrzykowska Malgorzata
Rantala Marjaana
Kangasjärvi Saijaliisa
Paakkarinen Virpi
Tikkanen Mikko
Jansson Stefan
Aro Eva-Mari
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.112.097162
Published
2012-07-00
Epub
2012-00-20
Pages
2934-48
Language
English
Region
England
NLM ID
9208688
PMCID
PMC3426124
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.112.097162 PMC Full Text View journal details
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