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PMID: 22822206 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Arabidopsis mediator subunit MED25 differentially regulates jasmonate and abscisic acid signaling through interacting with the MYC2 and ABI5 transcription factors.

The Plant cell ·Vol. 24 ·No. 7 ·2012-07-00 ·Pages 2898-916

Chen R, Jiang H, Li L, Zhai Q, Qi L, Zhou W, Liu X, Li H, Zheng W, Sun J, Li C

Abstract

Transcriptional regulation plays a central role in plant hormone signaling. At the core of transcriptional regulation is the Mediator, an evolutionarily conserved, multisubunit complex that serves as a bridge between gene-specific transcription factors and the RNA polymerase machinery to regulate transcription. Here, we report the action mechanisms of the MEDIATOR25 (MED25) subunit of the Arabidopsis thaliana Mediator in regulating jasmonate- and abscisic acid (ABA)-triggered gene transcription. We show that during jasmonate signaling, MED25 physically associates with the basic helix-loop-helix transcription factor MYC2 in promoter regions of MYC2 target genes and exerts a positive effect on MYC2-regulated gene transcription. We also show that MED25 physically associates with the basic Leu zipper transcription factor ABA-INSENSITIVE5 (ABI5) in promoter regions of ABI5 target genes and shows a negative effect on ABI5-regulated gene transcription. Our results reveal that underlying the distinct effects of MED25 on jasmonate and ABA signaling, the interaction mechanisms of MED25 with MYC2 and ABI5 are different. These results highlight that the MED25 subunit of the Arabidopsis Mediator regulates a wide range of signaling pathways through selectively interacting with specific transcription factors.

MeSH Terms
Abscisic Acid/metabolism Arabidopsis/cytology,genetics,physiology Arabidopsis Proteins/genetics,metabolism Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/genetics,metabolism Basic-Leucine Zipper Transcription Factors/genetics,metabolism Cyclopentanes/metabolism DNA-Binding Proteins Flowers/cytology,genetics,physiology Gene Expression Regulation, Plant/physiology Germination Mutation Nuclear Proteins/genetics,metabolism Oxylipins/metabolism Plant Growth Regulators/metabolism Plant Leaves/cytology,genetics,physiology Plant Roots/cytology,genetics,physiology Plants, Genetically Modified Promoter Regions, Genetic Protein Interaction Mapping Protein Structure, Tertiary Seedlings/cytology,genetics,physiology Seeds/cytology,genetics,physiology Signal Transduction/physiology Time Factors
Chemicals
ABI5 protein, Arabidopsis Arabidopsis Proteins Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Basic-Leucine Zipper Transcription Factors Cyclopentanes DNA-Binding Proteins MYC2 protein, Arabidopsis Nuclear Proteins Oxylipins PFT1 protein, Arabidopsis Plant Growth Regulators jasmonic acid Abscisic Acid
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Chen Rong
State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Jiang Hongling
Li Lin
Zhai Qingzhe
Qi Linlin
Zhou Wenkun
Liu Xiaoqiang
Li Hongmei
Zheng Wenguang
Sun Jiaqiang
Li Chuanyou
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.112.098277
Published
2012-07-00
Epub
2012-00-20
Pages
2898-916
Language
English
Region
England
NLM ID
9208688
PMCID
PMC3426122
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.112.098277 PMC Full Text View journal details
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