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PMID: 228295 Published · ppublish English Journal Article

A general priming system employing only dnaB protein and primase for DNA replication.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 9 ·1979-09-00 ·Pages 4308-12

Arai K, Kornberg A

Abstract

Priming of phage phi X174 DNA synthesis is effected simply by dnaB protein and primase when the DNA is not coated by single-strand binding protein (SSB). The five prepriming proteins (n,n',n'',i, and dnaC protein) required for priming a SSB-coated phi X174 DNA circle are dispensable. The dnaB protein-primase priming system is also active on uncoated phage G4 and M13 DNAs and on poly(dT). Multiple RNA primers, 10--60 nucleotides long, are transcribed with patterns distinctive for each DNA template. Formation of a stable dnaB protein.DNA complex in the presence of primase and ATP supports the hypothesis that dnaB protein provides a mobile replication promoter signal for primase.

MeSH Terms
Bacterial Proteins/metabolism Bacteriophage phi X 174/genetics Coliphages/genetics DNA Helicases/metabolism DNA Polymerase III/metabolism DNA Replication DNA, Single-Stranded/metabolism DNA, Viral/metabolism Escherichia coli/genetics Poly T/metabolism Templates, Genetic Virus Replication
Chemicals
Bacterial Proteins DNA, Single-Stranded DNA, Viral Poly T DNA Polymerase III DNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Arai K
Kornberg A
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.9.4308
Published
1979-09-00
Pages
4308-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411563
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.9.4308 PMC Full Text View journal details
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