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PMID: 22995226 Published · epublish English Journal Article

Extrinsic and intrinsic regulation of DOR/TP53INP2 expression in mice: effects of dietary fat content, tissue type and sex in adipose and muscle tissues.

Nutrition & metabolism ·Vol. 9 ·No. 1 ·2012-09-21 ·Pages 86

Fromm-Dornieden C, Lytovchenko O, von der Heyde S, Behnke N, Hogl S, Berghoff J, Köpper F, Opitz L, Renne U, Hoeflich A, Beissbarth T, Brenig B, Baumgartner BG

Abstract

DOR/TP53INP2 acts both at the chromosomal level as a nuclear co-factor e.g. for the thyroid hormone receptor and at the extrachromosomal level as an organizing factor of the autophagosome. In a previous study, DOR was shown to be down-regulated in skeletal muscle of obese diabetic Zucker fa/fa rats. To identify sites of differential DOR expression in metabolically active tissues, we measured differences in DOR expression in white adipose tissue (WAT), brown adipose tissue (BAT), skeletal muscle (SM) and heart muscle (HM) by qPCR. To assess whether DOR expression is influenced in the short term by nutritional factors, NMRI mice were fed different fat rich diets (fat diet, FD: 18% or high fat diet, HFD: 80% fat) for one week and DOR expression was compared to NMRI mice fed a control diet (normal diet, ND: 3.3% fat). Additionally, DOR expression was measured in young (45 days old) and adult (100 days old) genetically obese (DU6/DU6i) mice and compared to control (DUKs/DUKsi) animals. ANOVA results demonstrate a significant influence of diet, tissue type and sex on DOR expression in adipose and muscle tissues of FD and HFD mice. In SM, DOR expression was higher in HFD than in FD male mice. In WAT, DOR expression was increased compared to BAT in male FD and HFD mice. In contrast, expression levels in female mice were higher in BAT for both dietary conditions.DOR expression levels in all tissues of 100 days old genetically obese animals were mainly influenced by sex. In HM, DOR expression was higher in male than female animals. DOR expression varies under the influence of dietary fat content, tissue type and sex. We identified target tissues for further studies to analyze the specific function of DOR in obesity. DOR might be part of a defense mechanism against fat storage in high fat diets or obesity.

Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Fromm-Dornieden Carolin
Institute of Veterinary Medicine, University of Göttingen, Burckhardtweg 2, 37077, Göttingen, Germany.
Lytovchenko Oleksandr
Institute of Veterinary Medicine, University of Göttingen, Burckhardtweg 2, 37077, Göttingen, Germany.
von der Heyde Silvia
Statistical Bioinformatics, Department of Medical Statistics, University Medical Center Göttingen, Humboldtallee 32, 37073, Göttingen, Germany.
Behnke Nina
Institute of Veterinary Medicine, University of Göttingen, Burckhardtweg 2, 37077, Göttingen, Germany.
Hogl Sebastian
Institute of Veterinary Medicine, University of Göttingen, Burckhardtweg 2, 37077, Göttingen, Germany.
Berghoff Janina
Institute of Veterinary Medicine, University of Göttingen, Burckhardtweg 2, 37077, Göttingen, Germany.
Köpper Frederik
Institute of Veterinary Medicine, University of Göttingen, Burckhardtweg 2, 37077, Göttingen, Germany.
Opitz Lennart
DNA Microarray Facility, Department of Developmental Biochemistry, University of Göttingen, Humboldtallee 23, 37073, Göttingen, Germany.
Renne Ulla
Research Units Genetics & Biometry, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
Hoeflich Andreas
Research Units Genetics & Biometry, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
Beissbarth Tim
Statistical Bioinformatics, Department of Medical Statistics, University Medical Center Göttingen, Humboldtallee 32, 37073, Göttingen, Germany.
Brenig Bertram
Institute of Veterinary Medicine, University of Göttingen, Burckhardtweg 2, 37077, Göttingen, Germany.
Baumgartner Bernhard G
Institute of Veterinary Medicine, University of Göttingen, Burckhardtweg 2, 37077, Göttingen, Germany. | Department of Internal Medicine, Metabolic Diseases and Medical Molecular Biology, Paracelsus Private Medical University Salzburg, Müllner Hauptstr. 48, 5020, Salzburg, Austria.
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Article Info
Journal
Nutrition & metabolism
Abbr.
Nutr Metab (Lond)
ISSN
1743-7075
DOI
10.1186/1743-7075-9-86
Published
2012-09-21
Epub
2012-00-21
Pages
86
Language
English
Region
England
NLM ID
101231644
PMCID
PMC3497704
External Links
PubMed source DOI: 10.1186/1743-7075-9-86 PMC Full Text View journal details
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