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PMID: 2303041 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Amino-terminal domain of NF1 binds to DNA as a dimer and activates adenovirus DNA replication.

The EMBO journal ·Vol. 9 ·No. 2 ·1990-02-00 ·Pages 559-66

Gounari F, De Francesco R, Schmitt J, van der Vliet P, Cortese R, Stunnenberg H

Abstract

NF1 is a DNA-binding protein involved in initiation of adenovirus DNA replication as well as in modulating the rate of transcription initiation of genes containing the sequence TGGCA. We show here that recombinant NF1 expressed via vaccinia virus is transported into the nucleus and binds to its cognate sequences with the same specificity as NF1 purified from HeLa cells. Furthermore, the recombinant NF1 forms oligomers in solution and binds as a dimer to palindromic as well as half-site sequences. NF1 expressed via vaccinia virus stimulates the initiation of adenovirus replication in vitro. The N-terminal 240 amino acids of the protein are sufficient for full DNA-binding activity as well as stimulation of adenovirus replication. By analysis of several NF1 mutants translated in vitro, we also define the minimal DNA-binding domain and localize the region responsible for DNA binding on the N-terminal and for oligomerization on the C-terminal side of this domain.

MeSH Terms
Adenoviruses, Human/genetics Amino Acid Sequence Animals Base Sequence Binding, Competitive CCAAT-Enhancer-Binding Proteins DNA Replication DNA, Viral/genetics,metabolism DNA-Binding Proteins/genetics,metabolism HeLa Cells/metabolism Humans Kinetics Macromolecular Substances Molecular Sequence Data NFI Transcription Factors Nuclear Proteins Oligonucleotide Probes Plasmids Protein Biosynthesis Recombinant Proteins/metabolism Recombination, Genetic Transcription Factors Transcription, Genetic Vaccinia virus/genetics Y-Box-Binding Protein 1
Chemicals
CCAAT-Enhancer-Binding Proteins DNA, Viral DNA-Binding Proteins Macromolecular Substances NFI Transcription Factors Nuclear Proteins Oligonucleotide Probes Recombinant Proteins Transcription Factors Y-Box-Binding Protein 1 YBX1 protein, human
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gounari F
European Molecular Biology Laboratory, Heidelberg, FRG.
De Francesco R
Schmitt J
van der Vliet P
Cortese R
Stunnenberg H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-02-00
Pages
559-66
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551701
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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