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PMID: 2308839 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Structure and transcription of a human gene for H1 RNA, the RNA component of human RNase P.

Nucleic acids research ·Vol. 18 ·No. 1 ·1990-01-11 ·Pages 97-103

Baer M, Nilsen TW, Costigan C, Altman S

Abstract

The gene coding for H1 RNA, the RNA component of human RNase P, has been isolated and characterized from a human genomic DNA library. The sequence corresponding to the mature H1 RNA is almost identical to that previously identified using H1 RNA and a cDNA clone corresponding to it. The nucleotide sequence of the genomic clone contains an array of potential transcriptional control elements, some characteristic of transcription by RNA polymerase III and some characteristic of RNA polymerase II, as is also the case for U6 and certain other small stable RNAs. The transcription in vitro of the genomic clone shows that the gene is functional and is transcribed by RNA polymerase III. Southern hybridization analysis indicates that there is very likely only one copy of the gene for H1 RNA in the human genome.

MeSH Terms
Base Sequence Chromosome Mapping Chromosomes, Human, Pair 14 Cloning, Molecular Endoribonucleases/genetics Gene Expression Regulation Genes Humans Molecular Sequence Data Promoter Regions, Genetic RNA Polymerase III/metabolism Regulatory Sequences, Nucleic Acid Ribonuclease P Transcription, Genetic
Chemicals
RNA Polymerase III Endoribonucleases RPP14 protein, human Ribonuclease P
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Baer M
Department of Biology, Yale University, New Haven, CT 06520.
Nilsen T W
Costigan C
Altman S
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.1.97
Published
1990-01-11
Pages
97-103
Language
English
Region
England
NLM ID
0411011
PMCID
PMC330208
Subset
IM
Grants
NIGMS NIH HHS · GM-19422 · United States
NIGMS NIH HHS · GM-31528 · United States
External Links
PubMed source DOI: 10.1093/nar/18.1.97 PMC Full Text View journal details
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