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PMID: 23194270 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

Sperm phosphoproteomics: historical perspectives and current methodologies.

Expert review of proteomics ·Vol. 9 ·No. 5 ·2012-10-00 ·Pages 533-48

Porambo JR, Salicioni AM, Visconti PE, Platt MD

Abstract

Mammalian sperm are differentiated germ cells that transfer genetic material from the male to the female. Owing to this essential role in the reproductive process, an understanding of the complex mechanisms that underlie sperm function has implications ranging from the development of novel contraceptives to the treatment of male infertility. While the importance of phosphorylation in sperm differentiation, maturation and fertilization has been well established, the ability to directly determine the sites of phosphorylation within sperm proteins and to quantitate the extent of phosphorylation at these sites is a recent development that has relied almost exclusively on advances in the field of proteomics. This review will summarize the work that has been carried out to date on sperm phosphoproteomics and discuss how the resulting qualitative and quantitative information has been used to provide insight into the manner in which protein phosphorylation events modulate sperm function. The authors also present the proteomics process as it is most often utilized for the elucidation of protein expression, with a particular emphasis on the way in which the process has been modified for the analysis of protein phosphorylation in sperm.

MeSH Terms
Fertilization Gene Expression Regulation Humans Infertility, Male/metabolism,pathology,therapy Male Mass Spectrometry Phosphoproteins/chemistry,metabolism Phosphorylation Proteomics/methods Spermatozoa/chemistry,metabolism
Chemicals
Phosphoproteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Porambo James R
Department of Chemistry and Chemical Biology, Troy, NY 12180-3590, USA.
Salicioni Ana M
Visconti Pablo E
Platt Mark D
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Article Info
Journal
Expert review of proteomics
Abbr.
Expert Rev Proteomics
ISSN
1744-8387
DOI
10.1586/epr.12.41
Published
2012-10-00
Pages
533-48
Language
English
Region
England
NLM ID
101223548
PMCID
PMC3561639
Subset
IM
Grants
NICHD NIH HHS · R01 HD038082 · United States
NICHD NIH HHS · R01 HD044044 · United States
NICHD NIH HHS · HD38082 · United States
NICHD NIH HHS · HD44044 · United States
External Links
PubMed source DOI: 10.1586/epr.12.41 PMC Full Text View journal details
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