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PMID: 21540868 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

Ion channels, phosphorylation and mammalian sperm capacitation.

Asian journal of andrology ·Vol. 13 ·No. 3 ·2011-05-00 ·Pages 395-405

Visconti PE, Krapf D, de la Vega-Beltrán JL, Acevedo JJ, Darszon A

Abstract

Sexually reproducing animals require an orchestrated communication between spermatozoa and the egg to generate a new individual. Capacitation, a maturational complex phenomenon that occurs in the female reproductive tract, renders spermatozoa capable of binding and fusing with the oocyte, and it is a requirement for mammalian fertilization. Capacitation encompasses plasma membrane reorganization, ion permeability regulation, cholesterol loss and changes in the phosphorylation state of many proteins. Novel tools to study sperm ion channels, image intracellular ionic changes and proteins with better spatial and temporal resolution, are unraveling how modifications in sperm ion transport and phosphorylation states lead to capacitation. Recent evidence indicates that two parallel pathways regulate phosphorylation events leading to capacitation, one of them requiring activation of protein kinase A and the second one involving inactivation of ser/thr phosphatases. This review examines the involvement of ion transporters and phosphorylation signaling processes needed for spermatozoa to achieve capacitation. Understanding the molecular mechanisms leading to fertilization is central for societies to deal with rising male infertility rates, to develop safe male gamete-based contraceptives and to preserve biodiversity through better assisted fertilization strategies.

MeSH Terms
Animals Calcium/metabolism Calcium Channels Cyclic AMP-Dependent Protein Kinases/metabolism Cystic Fibrosis Transmembrane Conductance Regulator/physiology Epithelial Sodium Channels/physiology Female Fertilization Hydrogen-Ion Concentration Ion Channels/physiology Male Membrane Microdomains/physiology Phospholipid Transfer Proteins/physiology Phosphoprotein Phosphatases/metabolism Phosphorylation Potassium Channels, Inwardly Rectifying/physiology Signal Transduction/physiology Sperm Capacitation/physiology Spermatozoa/physiology src-Family Kinases/physiology
Chemicals
Calcium Channels Catsper1 protein, mouse Epithelial Sodium Channels Ion Channels Phospholipid Transfer Proteins Potassium Channels, Inwardly Rectifying Cystic Fibrosis Transmembrane Conductance Regulator src-Family Kinases Cyclic AMP-Dependent Protein Kinases Phosphoprotein Phosphatases Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Visconti Pablo E
Department of Veterinary and Animal Science, Paige Labs, University of Massachusets, Amherst, MA 01003, USA.
Krapf Dario
de la Vega-Beltrán José Luis
Acevedo Juan José
Darszon Alberto
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Article Info
Journal
Asian journal of andrology
Abbr.
Asian J Androl
ISSN
1745-7262
DOI
10.1038/aja.2010.69
Published
2011-05-00
Pages
395-405
Language
English
Region
China
NLM ID
100942132
PMCID
PMC3739340
Subset
IM
Grants
NICHD NIH HHS · R01 HD038082 · United States
NICHD NIH HHS · R01 HD044044 · United States
NICHD NIH HHS · R01 HD44044 · United States
External Links
PubMed source DOI: 10.1038/aja.2010.69 PMC Full Text View journal details
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