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PMID: 19860887 Published · epublish English Journal Article Research Support, N.I.H., Extramural

The BSA-induced Ca2+ influx during sperm capacitation is CATSPER channel-dependent.

Reproductive biology and endocrinology : RB&E ·Vol. 7 ·2009-10-27 ·Pages 119

Xia J, Ren D

Abstract

Serum albumin is a key component in mammalian sperm capacitation, a functional maturation process by which sperm become competent to fertilize oocytes. Capacitation is accompanied by several cellular and molecular changes including an increased tyrosine phosphorylation of sperm proteins and a development of hyperactivated sperm motility. Both of these processes require extracellular calcium, but how calcium enters sperm during capacitation is not well understood. BSA-induced changes in intracellular calcium concentration were studied using Fluo-4 and Fura-2 calcium imaging with wild-type and Catsper1 knockout mouse sperm. We found that the fast phase of the BSA-induced rises in intracellular calcium concentration was absent in the Catsper1 knockout sperm and could be restored by an EGFP-CATSPER1 fusion protein. The calcium concentration increases were independent of G-proteins and phospholipase C but could be partially inhibited when intracellular pH was clamped. The changes started in the principal piece and propagated toward the sperm head. We conclude that the initial phase of the increases in intracellular calcium concentration induced by BSA requires the CATSPER channel, but not the voltage-gated calcium channel. Our findings identify the molecular conduit responsible for the calcium entry required for the sperm motility changes that occur during capacitation.

MeSH Terms
Adenosine Triphosphate/pharmacology Animals Calcium/metabolism Calcium Channels/genetics,physiology Calcium Signaling/drug effects Hydrogen-Ion Concentration Male Mice Mice, Inbred C57BL Mice, Transgenic Serum Albumin, Bovine/pharmacology Sperm Capacitation/drug effects,genetics Sperm Motility/drug effects,genetics Spermatozoa/drug effects,metabolism Tissue Distribution/drug effects
Chemicals
Calcium Channels Catsper1 protein, mouse Serum Albumin, Bovine Adenosine Triphosphate Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Xia Jingsheng
Department of Biology, University of Pennsylvania, 415 S University Ave, Philadelphia, Pennsylvania 19104, USA.
Ren Dejian
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Article Info
Journal
Reproductive biology and endocrinology : RB&E
Abbr.
Reprod Biol Endocrinol
ISSN
1477-7827
DOI
10.1186/1477-7827-7-119
Published
2009-10-27
Epub
2009-00-27
Pages
119
Language
English
Region
England
NLM ID
101153627
PMCID
PMC2775032
Subset
IM
Grants
NICHD NIH HHS · R01 HD047578 · United States
NICHD NIH HHS · 1R01HD047578 · United States
External Links
PubMed source DOI: 10.1186/1477-7827-7-119 PMC Full Text View journal details
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