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PMID: 232177 Published · ppublish English Journal Article

Human cytomegalovirus-induced immediate early antigens: analysis in sodium dodecyl sulfate-polyacrylamide gel electrophoresis after immunoprecipitation.

Journal of virology ·Vol. 32 ·No. 1 ·1979-10-00 ·Pages 259-67

Michelson S, Horodniceanu F, Kress M, Tardy-Panit M

Abstract

Immediate early antigen (IEA) induced in human lung fibroblasts by human cytomegalovirus was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis after immunoprecipitation with IEA-positive human sera. Two polypeptides of 76,000 daltons (76K) and 82K were detectable within 90 min after infection. Polypeptides of similar molecular weight were also found in immunoprecipitates of human cytomegalovirus-infected cells nonpermissive for virus replication. IEA is located within the nucleus, although some of the 76K material appears to be located on the outer nuclear membrane. Raising salt concentrations in the extraction buffer increased antigen extraction. The contribution of these IEA polypeptides to IEA nuclear fluorescent staining is discussed.

MeSH Terms
Animals Antigen-Antibody Reactions Antigens, Viral/analysis Cell Line Chemical Precipitation Cycloheximide/pharmacology Cytomegalovirus/immunology Dactinomycin/pharmacology Electrophoresis, Polyacrylamide Gel Humans Lung Peptides/analysis Rabbits Skin Viral Proteins/analysis
Chemicals
Antigens, Viral Peptides Viral Proteins Dactinomycin Cycloheximide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Michelson S
Horodniceanu F
Kress M
Tardy-Panit M
References (19)
19 references, click to expand
  1. Human cytomegalovirus stimulates host cell RNA synthesis.
    J Virol. 1975 Feb;15(2):297-304 PMID: 163357
  2. Modification of simian virus 40 protein A.
    J Virol. 1977 Feb;21(2):647-57 PMID: 189092
  3. Evidence for early nuclear antigens in cytomegalovirus-infected cells.
    J Gen Virol. 1976 Aug;32(2):315-9 PMID: 190342
  4. Synthesis of proteins and glycoproteins in cells infected with human cytomegalovirus.
    J Virol. 1977 Sep;23(3):751-67 PMID: 197270
  5. Growth of cytomegalovirus at supra-optimal temperatures.
    J Gen Virol. 1977 Sep;36(3):437-47 PMID: 199693
  6. Cytomegalovirus-induced membrane antigens in productively infected cells.
    Acta Microbiol Acad Sci Hung. 1977;24(1):21-8 PMID: 201154
  7. Immediate early antigens in human cytomegalovirus infected cells.
    Nature. 1977 Dec 15;270(5638):615-7 PMID: 201863
  8. Sequence of protein synthesis in cells infected by human cytomegalovirus: early and late virus-induced polypeptides.
    J Virol. 1978 Jun;26(3):686-701 PMID: 209215
  9. Development of early nuclear antigen in cytomegalovirus infected cells in the presence of RNA and protein synthesis inhibitors.
    J Gen Virol. 1978 Aug;40(2):475-80 PMID: 211192
  10. Chromatin pattern consisting of repeating bipartite structures in WI-38 cells infected with human cytomegalovirus.
    J Virol. 1978 Nov;28(2):661-4 PMID: 214585
  11. Human cytomegalovirus-induced chromatin factors responsible for changes in template activity and structure of infected cell chromatin.
    Virology. 1978 Oct 15;90(2):197-208 PMID: 214942
  12. Comparison of occurrence of antibodies to human cytomegalovirus as demonstrated by immunofluorescence and indirect hemagglutination techniques.
    J Clin Microbiol. 1979 Jan;9(1):149-51 PMID: 219018
  13. Antibody reactions to virus-specific early antigens (EA) in patients with cytomegalovirus (CMV) infection.
    Clin Exp Immunol. 1974 Jan;16(1):1-7,9-12 PMID: 4143184
  14. The effect of cytosine-arabinoside on the multiplication of cytomegalovirus and on the formation of virus-induced intracellular antigens.
    Acta Virol. 1973 May;17(3):189-95 PMID: 4147136
  15. Characteristics of a human diploid cell designated MRC-5.
    Nature. 1970 Jul 11;227(5254):168-70 PMID: 4316953
  16. Growth characteristics of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication.
    J Virol. 1973 Jun;11(6):991-7 PMID: 4351464
  17. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  18. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  19. Stimulation of cellular DNA synthesis by human cytomegalovirus.
    J Virol. 1974 Feb;13(2):353-62 PMID: 16789142
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.32.1.259-267.1979
Published
1979-10-00
Pages
259-67
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353550
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.32.1.259-267.1979 PMC Full Text View journal details
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