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PMID: 232180 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Physical map of biologically active Harvey sarcoma virus unintegrated linear DNA.

Journal of virology ·Vol. 32 ·No. 1 ·1979-10-00 ·Pages 30-9

Goldfarb MP, Weinberg RA

Abstract

BALB/c JLS V9 cells recently infected with Harvey sarcoma virus-murine leukemia virus (HSV-MuLV) complex contained unintegrated HSV linear DNA of 6.0-kilobase pair mass. The cells also contained two HSV closed circular DNA species along with MuLV-encoded linear and closed circular DNA species. HSV 6.0-kilobase pair linear DNA induced focal transformation upon transfection of NIH 3T3 mouse fibroblasts, and the biological activity of HSV DNA did not require helper MuLV functions. A physical map of restriction endonuclease cleavage sites along HSV 6.0-kilobase pair linear DNA was derived. Comparison of this map with one for Moloney MuLV DNA showed that the HSV and Moloney MuLV genomes are identical near their viral RNA 3' ends.

MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral Chromosome Mapping DNA Restriction Enzymes/metabolism DNA, Circular/analysis DNA, Viral/analysis Fibroblasts Mice Sarcoma Viruses, Murine/analysis Transfection
Chemicals
DNA, Circular DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goldfarb M P
Weinberg R A
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29 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.32.1.30-39.1979
Published
1979-10-00
Pages
30-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353523
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.32.1.30-39.1979 PMC Full Text View journal details
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