Home LiteratureArticle Details
PMID: 2326188 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Human ureaplasmas show diverse genome sizes by pulsed-field electrophoresis.

Nucleic acids research ·Vol. 18 ·No. 6 ·1990-03-25 ·Pages 1451-5

Robertson JA, Pyle LE, Stemke GW, Finch LR

Abstract

Contour clamped homogeneous field (CHEF) agarose gel electrophoresis (AGE), ramped to give linear separation of DNA molecules of 600-1600 kilobase pairs (kbp), was used to determine mobilities for full-sized genomic DNA of the serotype standard strains of the human genital mollicutes, Ureaplasma urealyticum relative to yeast chromosomal DNA markers. Indicated genome sizes (in kbp) were 760 for the four biotype 1 strains and 840-1140 for eleven biotype 2 strains. Other estimates were: 720 for Mycoplasma hominis, 1070 for Mycoplasma hyopneumoniae, 890 for Mycoplasma flocculare, 1180 and 1350 for Mycoplasma mycoides subsp. mycoides Y and GC1176-2, respectively, and 1650 and 1580 for Acholeplasma laidlawii B and PG 8, respectively. These data supplement previous evidence from CHEF AGE that the genomes of the Mycoplasmataceae are diverse in size with some larger than previously estimated from DNA renaturation kinetics.

MeSH Terms
DNA, Bacterial/genetics,isolation & purification Electrophoresis, Agar Gel Genes, Bacterial Humans Molecular Weight Mycoplasma/genetics Restriction Mapping Serotyping Ureaplasma/genetics,isolation & purification
Chemicals
DNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Robertson J A
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
Pyle L E
Stemke G W
Finch L R
References (28)
28 references, click to expand
  1. Heterogeneity among the mycoplasma and relationships to bacteria.
    Ann N Y Acad Sci. 1967 Jul 28;143(1):31-7 PMID: 5233767
  2. Molecular genetic studies of relationships among mycoplasma, L-forms and bacteria.
    Ann N Y Acad Sci. 1967 Jul 28;143(1):21-30 PMID: 5233759
  3. Lack of deoxyribonucleic acid homology between species of the genus Chlamydia.
    J Bacteriol. 1968 Oct;96(4):1421-3 PMID: 5686006
  4. Partial purification of native rRNA and tRNA cistrons from mycoplasma sp. (Kid).
    Proc Natl Acad Sci U S A. 1969 Aug;63(4):1282-9 PMID: 5260931
  5. Genome size of mycoplasmal DNA.
    Nature. 1969 Dec 20;224(5225):1209-10 PMID: 5358346
  6. Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare a survey.
    Nord Vet Med. 1975 Jun;27(6):337-9 PMID: 1098011
  7. Bromothymol blue broth: improved medium for detection of Ureaplasma urealyticum (T-strain mycoplasma).
    J Clin Microbiol. 1978 Feb;7(2):127-32 PMID: 632344
  8. Modified metabolic inhibition test for serotyping strains of Ureaplasma urealyticum (T-strain Mycoplasma).
    J Clin Microbiol. 1979 Jun;9(6):673-6 PMID: 500800
  9. Comparison of human and animal Ureaplasmas by one- and two-dimensional protein analysis on polyacrylamide slab gel.
    Ann Microbiol (Paris). 1981 Sep-Oct;132B(2):171-96 PMID: 6798909
  10. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.
    Cell. 1984 May;37(1):67-75 PMID: 6373014
  11. Effects of manganese on the growth and morphology of Ureaplasma urealyticum.
    J Clin Microbiol. 1984 Jun;19(6):857-64 PMID: 6432835
  12. Characterization of the mycoplasma genome.
    Yale J Biol Med. 1983 Sep-Dec;56(5-6):357-66 PMID: 6206652
  13. Molecular biology and genetics of mycoplasmas (Mollicutes).
    Microbiol Rev. 1985 Dec;49(4):419-55 PMID: 3912655
  14. Separation of large DNA molecules by contour-clamped homogeneous electric fields.
    Science. 1986 Dec 19;234(4783):1582-5 PMID: 3538420
  15. Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields.
    Nucleic Acids Res. 1987 Oct 12;15(19):7865-76 PMID: 2959907
  16. Preparation and FIGE separation of infrequent restriction fragments from Mycoplasma mycoides DNA.
    Nucleic Acids Res. 1988 Mar 25;16(5):2263-8 PMID: 2833729
  17. Pulsed-field gel electrophoresis of very large DNA molecules.
    Annu Rev Biophys Biophys Chem. 1988;17:287-304 PMID: 3293589
  18. Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes.
    Nucleic Acids Res. 1988 Jul 11;16(13):6015-25 PMID: 2840639
  19. A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci.
    Nucleic Acids Res. 1988 Jul 11;16(13):6027-39 PMID: 2840640
  20. Homology of mycoplasma plasmid pADB201 and staphylococcal plasmid pE194.
    J Bacteriol. 1989 Jan;171(1):593-5 PMID: 2644210
  21. Improved control of partial DNA restriction enzyme digest in agarose using limiting concentrations of Mg++.
    Nucleic Acids Res. 1989 Jan 25;17(2):808 PMID: 2915934
  22. Anomalous values of Mycoplasma genomes sizes determined by pulse-field gel electrophoresis.
    Nucleic Acids Res. 1989 Feb 11;17(3):1268 PMID: 2922272
  23. High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 1. DNA size standards and the effect of agarose and temperature.
    Biochemistry. 1988 Dec 27;27(26):9204-10 PMID: 3072024
  24. Pulsed-field gel electrophoresis determination of the genome size of obligate intracellular bacteria belonging to the genera Chlamydia, Rickettsiella, and Porochlamydia.
    J Bacteriol. 1989 Aug;171(8):4511-3 PMID: 2753864
  25. A physical map of the genome of Ureaplasma urealyticum 960T with ribosomal RNA loci.
    Nucleic Acids Res. 1989 Aug 25;17(16):6713-9 PMID: 2780294
  26. Location of sites of transposon Tn916 insertion in the Mycoplasma mycoides genome.
    J Bacteriol. 1989 Dec;171(12):6870-2 PMID: 2556378
  27. Tissue cultures and mycoplasmas.
    Tex Rep Biol Med. 1965 Jun;23:Suppl 1:285+ PMID: 5833547
  28. DNA base composition of human T strain mycoplasmas.
    Nature. 1968 Sep 7;219(5158):1044-5 PMID: 5673359
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.6.1451
Published
1990-03-25
Pages
1451-5
Language
English
Region
England
NLM ID
0411011
PMCID
PMC330511
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.6.1451 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2