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PMID: 2328721 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of ras-related, YPT family genes in Schizosaccharomyces pombe.

The EMBO journal ·Vol. 9 ·No. 5 ·1990-05-00 ·Pages 1417-22

Miyake S, Yamamoto M

Abstract

Screening for genes homologous to ras in Schizosaccharomyces pombe resulted in the isolation of a homolog of Saccharomyces cerevisiae YPT1. This S. pombe gene, named ypt3, has a coding capacity of 214 amino acids interrupted by two introns, and is essential for cell growth. Two more YPT1 homologs were isolated from S. pombe using a part of the ypt3 gene as the probe. One of them, named ypt1, is highly homologous to S. cerevisiae YPT1 and mouse ypt1 and is essential for cell growth. This gene has four introns and encodes 203 amino acids. Its cDNA placed downstream of the S. cerevisiae GAL7 promoter could complement S. cerevisiae ypt1-, indicating that Sp ypt1 and Sc YPT1 are functionally homologous. The other isolate, named ryh1, and a fourth homolog, ypt2, have been characterized by Gallwitz and co-workers. The ypt1, ypt2 and ypt3 genes, but not ryh1, constitute a family, their products having double cysteine as their C terminus and serine in place of a glycine residue highly conserved in ras proteins (mammalian Gly-12 or S. pombe Gly-17). The physiological roles of these genes appear to be distinct because each of them is indispensable for cell growth.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Mutational Analysis Fungal Proteins/genetics Genetic Complementation Test Molecular Sequence Data Multigene Family Proteins/genetics Saccharomyces cerevisiae Proteins Saccharomycetales/genetics Schizosaccharomyces/genetics Sequence Homology, Nucleic Acid rab GTP-Binding Proteins
Chemicals
Fungal Proteins Proteins Saccharomyces cerevisiae Proteins YPT1 protein, S cerevisiae rab GTP-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Miyake S
Institute of Medical Science, University of Tokyo, Japan.
Yamamoto M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-05-00
Pages
1417-22
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551828
Subset
IM
Databases
GENBANK
X52099, X52100
External Links
PubMed source PMC Full Text View journal details
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