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PMID: 2670553 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The ras-related mouse ypt1 protein can functionally replace the YPT1 gene product in yeast.

The EMBO journal ·Vol. 8 ·No. 5 ·1989-05-00 ·Pages 1427-32

Haubruck H, Prange R, Vorgias C, Gallwitz D

Abstract

The protein-coding region of the essential Saccharomyces cerevisiae YPT1 gene coding for a ras-related, guanine-nucleotide-binding protein was exchanged in chromosome VI by the protein-coding segment of either the mouse ypt1 gene or the v-Ki-ras gene, and different chimeric YPT1-v-Ki-ras genes. The mouse ypt1 protein with 71% of identical residues compared with the yeast Ypt1 protein could functionally fully replace its yeast homologue as long as the mouse gene was overexpressed under transcriptional control of the inducible GAL10 promoter. In contrast, neither the viral Ki-ras nor the hybrid proteins were able to substitute for the loss of YPT1 gene function. This study suggests that different parts of the yeast Ypt1 protein are required for the interaction with cellular targets and that these essential parts are conserved in the mammalian ypt1 protein.

MeSH Terms
Amino Acid Sequence Animals Fungal Proteins/genetics Gene Expression Regulation Genes, Fungal Mice Molecular Sequence Data Oncogene Protein p21(ras) Oncogene Proteins, Viral/genetics Proteins/genetics Recombinant Proteins/genetics Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid Viral Proteins/genetics rab GTP-Binding Proteins
Chemicals
Fungal Proteins Oncogene Proteins, Viral Proteins Recombinant Proteins Saccharomyces cerevisiae Proteins Viral Proteins YPT1 protein, S cerevisiae Oncogene Protein p21(ras) rab GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Haubruck H
Max-Planck-Institut for Biophysical Chemistry, Department of Molecular Genetics, Göttingen, FRG.
Prange R
Vorgias C
Gallwitz D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-05-00
Pages
1427-32
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400970
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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