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PMID: 233905 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differences in pyrogen production by mononuclear phagocytes and by fibroblasts or HeLa cells.

The Journal of experimental medicine ·Vol. 145 ·No. 3 ·1977-03-01 ·Pages 607-17

Bodel P, Miller H

Abstract

Phagocytosis of bacteria stimulates "professional" phagocytes to produce and release endogenous pyrogen (EP), the protein that mediates fever. To determine whether "nonprofessional" phagocytes also have this capacity, mouse and human fibroblasts and HeLa cells were cultured after ingestion of latex or chicken erythrocytes (CE), and EP release into culture supernate measured by mouse assay. No detectable pyrogen was released by these cell types after phagocytosis, whereas both latex and CE stimulated EP production by cultured mouse macrophages. These studies support the hypothesis that only professional phagocytes of bone marrow origin synthesize EP and induce fever.

MeSH Terms
Animals Cells, Cultured Erythrocytes/metabolism Female Fibroblasts/metabolism HeLa Cells Humans L Cells Latex Male Mice Phagocytes/metabolism Phagocytosis Pyrogens/biosynthesis
Chemicals
Latex Pyrogens
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bodel P
Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.
Miller H
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.145.3.607
Published
1977-03-01
Pages
607-17
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2180710
Subset
IM
Grants
NIAID NIH HHS · AI 01564-20 · United States
NCI NIH HHS · CA 14566-03 · United States
External Links
PubMed source DOI: 10.1084/jem.145.3.607 PMC Full Text View journal details
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