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PMID: 23390961 Published · epublish English Journal Article Review

The sweet and sour of serological glycoprotein tumor biomarker quantification.

BMC medicine ·Vol. 11 ·2013-02-07 ·Pages 31

Kuzmanov U, Kosanam H, Diamandis EP

Abstract

Aberrant and dysregulated protein glycosylation is a well-established event in the process of oncogenesis and cancer progression. Years of study on the glycobiology of cancer have been focused on the development of clinically viable diagnostic applications of this knowledge. However, for a number of reasons, there has been only sparse and varied success. The causes of this range from technical to biological issues that arise when studying protein glycosylation and attempting to apply it to practical applications. This review focuses on the pitfalls, advances, and future directions to be taken in the development of clinically applicable quantitative assays using glycan moieties from serum-based proteins as analytes. Topics covered include the development and progress of applications of lectins, mass spectrometry, and other technologies towards this purpose. Slowly but surely, novel applications of established and development of new technologies will eventually provide us with the tools to reach the ultimate goal of quantification of the full scope of heterogeneity associated with the glycosylation of biomarker candidate glycoproteins in a clinically applicable fashion.

MeSH Terms
Animals Biomarkers, Tumor/blood,genetics Gene Expression Regulation, Neoplastic Glycoproteins/blood,genetics Glycosylation Humans Mass Spectrometry/methods,standards Neoplasms/blood,diagnosis,genetics
Chemicals
Biomarkers, Tumor Glycoproteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kuzmanov Uros
Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, 6th floor, 60 Murray Street, Box 32, Toronto, ON M5T 3L9, Canada.
Kosanam Hari
Diamandis Eleftherios P
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Article Info
Journal
BMC medicine
Abbr.
BMC Med
ISSN
1741-7015
DOI
10.1186/1741-7015-11-31
Published
2013-02-07
Epub
2013-00-07
Pages
31
Language
English
Region
England
NLM ID
101190723
PMCID
PMC3751898
Subset
IM
External Links
PubMed source DOI: 10.1186/1741-7015-11-31 PMC Full Text View journal details
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