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PMID: 10673297 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Altered mRNA expression of glycosyltransferases in human colorectal carcinomas and liver metastases.

Gut ·Vol. 46 ·No. 3 ·2000-03-00 ·Pages 359-66

Petretti T, Kemmner W, Schulze B, Schlag PM

Abstract

Biosynthesis of carbohydrate structures is tissue specific and developmentally regulated by glycosyltransferases such as fucosyltransferases, sialyltransferases, and N-acetylgluco- saminyltransferases. During carcinogenesis, aberrant glycosylation leads to the development of tumour subpopulations with different adhesion properties. Therefore alterations in glycosyltransferase mRNA expression in colorectal carcinomas were examined by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). Colorectal carcinoma specimens were classified and characterised according to the WHO/UICC system. Expression of fucosyltransferases FT-I, FT-III, FT-IV, FT-V, FT-VI, and FT-VII, sialyltransferases ST3Gal-I, ST3Gal-III, ST3Gal-IV, and ST6Gal-I, beta1,4-galacto- syltransferase, and beta1,6-Nacetylgluco- saminyltransferase V (GNT-V) was screened simultaneously in extracts of 22 homogenised tumour specimens by RT-PCR and compared with corresponding mucosa from each patient. Also 12 adenomas and 17 liver metastases of colorectal carcinomas were examined. GNT-V expression was enhanced in colorectal adenomas (p = 0.039), carcinomas (p<0.001), and liver metastases of colorectal carcinomas (p<0.001). Also, expression of fucosyltransferase FT-IV was increased in colorectal adenomas (p = 0.039) and carcinomas (p<0. 001). In addition, fucosyltransferase FT-I (p<0.001) and sialyltransferases ST6Gal-I (p = 0.004) and ST3Gal-III (p = 0.001) showed increased expression in carcinoma specimens. On the other hand, fucosyltransferase FT-III was less abundantly expressed in carcinomas exhibiting distant metastases (p = 0.046) and in highly invasive tumours (p = 0.041). Glycosyltransferase mRNA expression is significantly altered in colorectal adenomas and carcinomas isolated from surgical specimens. RT-PCR determination of specific glycosyltransferases may be helpful for earlier detection of carcinomas and for tumour prognosis.

MeSH Terms
Adult Aged Aged, 80 and over Colorectal Neoplasms/enzymology,genetics Female Gene Expression Glycosyltransferases/genetics,metabolism Humans Liver Neoplasms/enzymology,genetics,secondary Male Middle Aged Neoplasm Proteins/genetics,metabolism RNA, Messenger/metabolism Reverse Transcriptase Polymerase Chain Reaction Sialyltransferases/metabolism
Chemicals
Neoplasm Proteins RNA, Messenger Glycosyltransferases Sialyltransferases beta-D-galactoside alpha 2-6-sialyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Petretti T
Robert-Rössle-Klinik at the Max Delbrück Centre for Molecular Medicine, Department of Surgery and Surgical Oncology, Charité, Campus Buch, 13125 Berlin, Germany.
Kemmner W
Schulze B
Schlag P M
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Article Info
Journal
Gut
Abbr.
Gut
ISSN
0017-5749
DOI
10.1136/gut.46.3.359
Published
2000-03-00
Pages
359-66
Language
English
Region
England
NLM ID
2985108R
PMCID
PMC1727852
Subset
IM
External Links
PubMed source DOI: 10.1136/gut.46.3.359 PMC Full Text View journal details
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