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PMID: 23405139 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression profile of ectopic olfactory receptors determined by deep sequencing.

PloS one ·Vol. 8 ·No. 2 ·2013-00-00 ·Pages e55368

Flegel C, Manteniotis S, Osthold S, Hatt H, Gisselmann G

Abstract

Olfactory receptors (ORs) provide the molecular basis for the detection of volatile odorant molecules by olfactory sensory neurons. The OR supergene family encodes G-protein coupled proteins that belong to the seven-transmembrane-domain receptor family. It was initially postulated that ORs are exclusively expressed in the olfactory epithelium. However, recent studies have demonstrated ectopic expression of some ORs in a variety of other tissues. In the present study, we conducted a comprehensive expression analysis of ORs using an extended panel of human tissues. This analysis made use of recent dramatic technical developments of the so-called Next Generation Sequencing (NGS) technique, which encouraged us to use open access data for the first comprehensive RNA-Seq expression analysis of ectopically expressed ORs in multiple human tissues. We analyzed mRNA-Seq data obtained by Illumina sequencing of 16 human tissues available from Illumina Body Map project 2.0 and from an additional study of OR expression in testis. At least some ORs were expressed in all the tissues analyzed. In several tissues, we could detect broadly expressed ORs such as OR2W3 and OR51E1. We also identified ORs that showed exclusive expression in one investigated tissue, such as OR4N4 in testis. For some ORs, the coding exon was found to be part of a transcript of upstream genes. In total, 111 of 400 OR genes were expressed with an FPKM (fragments per kilobase of exon per million fragments mapped) higher than 0.1 in at least one tissue. For several ORs, mRNA expression was verified by RT-PCR. Our results support the idea that ORs are broadly expressed in a variety of tissues and provide the basis for further functional studies.

MeSH Terms
Adult Exons Female Gene Expression Profiling/methods High-Throughput Nucleotide Sequencing/methods Humans Middle Aged Olfactory Receptor Neurons/metabolism Pseudogenes RNA, Messenger/genetics Receptors, Odorant/genetics Signal Transduction Smell/genetics Transcriptome
Chemicals
RNA, Messenger Receptors, Odorant
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Flegel Caroline
Department of Cell Physiology, Ruhr-University Bochum, Bochum, Germany.
Manteniotis Stavros
Osthold Sandra
Hatt Hanns
Gisselmann Günter
References (82)
82 references, click to expand
  1. Activation state of the M3 muscarinic acetylcholine receptor modulates mammalian odorant receptor signaling.
    Sci Signal. 2011 Jan 11;4(155):ra1 PMID: 21224444
  2. The human vomeronasal type-1 receptor family--detection of volatiles and cAMP signaling in HeLa/Olf cells.
    FASEB J. 2008 May;22(5):1416-25 PMID: 18096815
  3. New GPCRs from a human lingual cDNA library.
    Chem Senses. 2001 Nov;26(9):1157-66 PMID: 11705801
  4. Recent advances in RNA sequence analysis.
    F1000 Biol Rep. 2010 Aug 19;2:64 PMID: 21173855
  5. G protein-coupled receptor signaling via Src kinase induces endogenous human transient receptor potential vanilloid type 6 (TRPV6) channel activation.
    J Biol Chem. 2011 Apr 15;286(15):13184-92 PMID: 21349844
  6. Enterochromaffin cells of the human gut: sensors for spices and odorants.
    Gastroenterology. 2007 May;132(5):1890-901 PMID: 17484882
  7. Global gene expression analysis of early response to chemotherapy treatment in ovarian cancer spheroids.
    BMC Genomics. 2008 Feb 26;9:99 PMID: 18302766
  8. Novel markers for enterochromaffin cells and gastrointestinal neuroendocrine carcinomas.
    Mod Pathol. 2009 Feb;22(2):261-72 PMID: 18953328
  9. Expression of Golf in the rat placenta: Possible implication in olfactory receptor transduction.
    Placenta. 2006 Jan;27(1):103-8 PMID: 16310044
  10. PSGR2, a novel G-protein coupled receptor, is overexpressed in human prostate cancer.
    Int J Cancer. 2006 Mar 15;118(6):1471-80 PMID: 16206286
  11. Tissue-specificity of prostate specific antigens: comparative analysis of transcript levels in prostate and non-prostatic tissues.
    Cancer Lett. 2006 May 18;236(2):229-38 PMID: 16046056
  12. Most "dark matter" transcripts are associated with known genes.
    PLoS Biol. 2010 May 18;8(5):e1000371 PMID: 20502517
  13. Identification of a testicular odorant receptor mediating human sperm chemotaxis.
    Science. 2003 Mar 28;299(5615):2054-8 PMID: 12663925
  14. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation.
    Nat Biotechnol. 2010 May;28(5):511-5 PMID: 20436464
  15. Olfactory receptor expressed in ganglia of the autonomic nervous system.
    J Neurosci Res. 2002 Apr 15;68(2):176-84 PMID: 11948663
  16. An integrative analysis of DNA methylation and RNA-Seq data for human heart, kidney and liver.
    BMC Syst Biol. 2011;5 Suppl 3:S4 PMID: 22784623
  17. Long noncoding RNAs with enhancer-like function in human cells.
    Cell. 2010 Oct 1;143(1):46-58 PMID: 20887892
  18. Expression of members of the putative olfactory receptor gene family in mammalian germ cells.
    Nature. 1992 Jan 30;355(6359):453-5 PMID: 1370859
  19. Chimeras taking shape: potential functions of proteins encoded by chimeric RNA transcripts.
    Genome Res. 2012 Jul;22(7):1231-42 PMID: 22588898
  20. High-throughput microarray detection of olfactory receptor gene expression in the mouse.
    Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14168-73 PMID: 15377787
  21. Odorant receptor expression in the mouse cerebral cortex.
    J Neurobiol. 2004 Feb 15;58(3):315-27 PMID: 14750145
  22. Identification of novel transcripts in annotated genomes using RNA-Seq.
    Bioinformatics. 2011 Sep 1;27(17):2325-9 PMID: 21697122
  23. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks.
    Nat Protoc. 2012 Mar 01;7(3):562-78 PMID: 22383036
  24. Molecular cloning and chromosomal mapping of olfactory receptor genes expressed in the male germ line: evidence for their wide distribution in the human genome.
    Biochem Biophys Res Commun. 1997 Aug 18;237(2):283-7 PMID: 9268701
  25. Olfactory-like receptor cDNAs are present in human lingual cDNA libraries.
    Biochem Biophys Res Commun. 2005 Jul 22;333(1):264-72 PMID: 15950192
  26. Two novel odorant receptor families expressed in spermatids undergo 5'-splicing.
    J Biol Chem. 1998 Apr 17;273(16):9378-87 PMID: 9545261
  27. Genomic structure and transcription of a murine odorant receptor gene: differential initiation of transcription in the olfactory and testicular cells.
    Biochem Biophys Res Commun. 1996 Apr 16;221(2):240-7 PMID: 8619840
  28. Functional expression of the olfactory signaling system in the kidney.
    Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):2059-64 PMID: 19174512
  29. A novel multigene family may encode odorant receptors: a molecular basis for odor recognition.
    Cell. 1991 Apr 5;65(1):175-87 PMID: 1840504
  30. Multiple controlling mechanisms of FGF1 gene expression through multiple tissue-specific promoters.
    Prog Nucleic Acid Res Mol Biol. 2001;70:155-74 PMID: 11642361
  31. Sequence analysis in the olfactory receptor gene cluster on human chromosome 17: recombinatorial events affecting receptor diversity.
    Genomics. 1996 Oct 15;37(2):147-60 PMID: 8921386
  32. Characterization of clustered MHC-linked olfactory receptor genes in human and mouse.
    Genome Res. 2001 Apr;11(4):519-30 PMID: 11282967
  33. The complete human olfactory subgenome.
    Genome Res. 2001 May;11(5):685-702 PMID: 11337468
  34. Evolution of odorant receptors expressed in mammalian testes.
    Genetics. 2000 Oct;156(2):785-97 PMID: 11014824
  35. Taste receptor signaling in the mammalian gut.
    Curr Opin Pharmacol. 2007 Dec;7(6):557-62 PMID: 18024184
  36. Functional expression of a mammalian odorant receptor.
    Science. 1998 Jan 9;279(5348):237-42 PMID: 9422698
  37. ReCount: a multi-experiment resource of analysis-ready RNA-seq gene count datasets.
    BMC Bioinformatics. 2011 Nov 16;12:449 PMID: 22087737
  38. Alternative isoform regulation in human tissue transcriptomes.
    Nature. 2008 Nov 27;456(7221):470-6 PMID: 18978772
  39. Possible roles for products of polymorphic MHC and linked olfactory receptor genes during selection processes in reproduction.
    Am J Reprod Immunol. 2002 Jul;48(1):34-42 PMID: 12322894
  40. The functional involvement of gut-expressed sweet taste receptors in glucose-stimulated secretion of glucagon-like peptide-1 (GLP-1) and peptide YY (PYY).
    Clin Nutr. 2011 Aug;30(4):524-32 PMID: 21324568
  41. Ric-8B promotes functional expression of odorant receptors.
    Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9310-4 PMID: 16754875
  42. Widespread ectopic expression of olfactory receptor genes.
    BMC Genomics. 2006 May 22;7:121 PMID: 16716209
  43. PSGR, a novel prostate-specific gene with homology to a G protein-coupled receptor, is overexpressed in prostate cancer.
    Cancer Res. 2000 Dec 1;60(23):6568-72 PMID: 11118034
  44. 7E olfactory receptor gene clusters and evolutionary chromosome rearrangements.
    Cytogenet Genome Res. 2006;112(1-2):6-10 PMID: 16276084
  45. Identification of a novel G-protein coupled receptor expressed in distinct brain regions and a defined olfactory zone.
    Recept Channels. 1998;6(2):141-51 PMID: 9932290
  46. A novel brain receptor is expressed in a distinct population of olfactory sensory neurons.
    Eur J Neurosci. 2000 Nov;12(11):3926-34 PMID: 11069588
  47. The transcriptional landscape of the yeast genome defined by RNA sequencing.
    Science. 2008 Jun 6;320(5881):1344-9 PMID: 18451266
  48. RNA-Seq: a revolutionary tool for transcriptomics.
    Nat Rev Genet. 2009 Jan;10(1):57-63 PMID: 19015660
  49. Complex transcription and splicing of odorant receptor genes.
    J Biol Chem. 2003 May 30;278(22):19691-701 PMID: 12637542
  50. The molecular receptive ranges of human TAS2R bitter taste receptors.
    Chem Senses. 2010 Feb;35(2):157-70 PMID: 20022913
  51. TopHat: discovering splice junctions with RNA-Seq.
    Bioinformatics. 2009 May 1;25(9):1105-11 PMID: 19289445
  52. Chemosensory Ca2+ dynamics correlate with diverse behavioral phenotypes in human sperm.
    J Biol Chem. 2011 May 13;286(19):17311-25 PMID: 21454470
  53. Initiation of translation in prokaryotes and eukaryotes.
    Gene. 1999 Jul 8;234(2):187-208 PMID: 10395892
  54. Genome regulation by long noncoding RNAs.
    Annu Rev Biochem. 2012;81:145-66 PMID: 22663078
  55. The genomic structure of human olfactory receptor genes.
    Genomics. 2000 Nov 15;70(1):49-61 PMID: 11087661
  56. The native rat olfactory cyclic nucleotide-gated channel is composed of three distinct subunits.
    J Neurosci. 1999 Jul 1;19(13):5332-47 PMID: 10377344
  57. Deorphanization of Dresden G protein-coupled receptor for an odorant receptor.
    J Recept Signal Transduct Res. 2007;27(4):323-34 PMID: 17885925
  58. Bitter taste receptors on airway smooth muscle bronchodilate by localized calcium signaling and reverse obstruction.
    Nat Med. 2010 Nov;16(11):1299-304 PMID: 20972434
  59. Analysis of BMP4 and BMP7 signaling in breast cancer cells unveils time-dependent transcription patterns and highlights a common synexpression group of genes.
    BMC Med Genomics. 2011 Nov 25;4:80 PMID: 22118688
  60. Mapping and quantifying mammalian transcriptomes by RNA-Seq.
    Nat Methods. 2008 Jul;5(7):621-8 PMID: 18516045
  61. Analysis of cancer metabolism with high-throughput technologies.
    BMC Bioinformatics. 2011 Oct 18;12 Suppl 10:S8 PMID: 22166000
  62. Characterizing the expression of the human olfactory receptor gene family using a novel DNA microarray.
    Genome Biol. 2007;8(5):R86 PMID: 17509148
  63. Change in mRNA Expression after Atenolol, a Beta-adrenergic Receptor Antagonist and Association with Pharmacological Response.
    Arch Drug Inf. 2009 Sep;2(3):41-50 PMID: 19915711
  64. The olfactory receptor gene superfamily: data mining, classification, and nomenclature.
    Mamm Genome. 2000 Nov;11(11):1016-23 PMID: 11063259
  65. An abundance of ubiquitously expressed genes revealed by tissue transcriptome sequence data.
    PLoS Comput Biol. 2009 Dec;5(12):e1000598 PMID: 20011106
  66. Transcriptomes of mouse olfactory epithelium reveal sexual differences in odorant detection.
    Genome Biol Evol. 2012;4(5):703-12 PMID: 22511034
  67. Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca²⁺ and cAMP concentrations in spermatozoa.
    PLoS One. 2012;7(2):e32354 PMID: 22427794
  68. Trace amines: identification of a family of mammalian G protein-coupled receptors.
    Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):8966-71 PMID: 11459929
  69. Generation and characterization of T1R2-LacZ knock-in mouse.
    Biochem Biophys Res Commun. 2010 Nov 19;402(3):495-9 PMID: 20965149
  70. How the olfactory system makes sense of scents.
    Nature. 2001 Sep 13;413(6852):211-8 PMID: 11557990
  71. The Sequence Alignment/Map format and SAMtools.
    Bioinformatics. 2009 Aug 15;25(16):2078-9 PMID: 19505943
  72. TRIM proteins and cancer.
    Nat Rev Cancer. 2011 Oct 07;11(11):792-804 PMID: 21979307
  73. Olfactory receptor gene cluster on human chromosome 17: possible duplication of an ancestral receptor repertoire.
    Hum Mol Genet. 1994 Feb;3(2):229-35 PMID: 8004088
  74. A signature of evolutionary constraint on a subset of ectopically expressed olfactory receptor genes.
    Mol Biol Evol. 2009 Mar;26(3):491-4 PMID: 19103638
  75. An olfactory receptor gene is located in the extended human beta-globin gene cluster and is expressed in erythroid cells.
    Genomics. 1999 Oct 1;61(1):15-23 PMID: 10512676
  76. Specific repertoire of olfactory receptor genes in the male germ cells of several mammalian species.
    Genomics. 1997 Feb 1;39(3):239-46 PMID: 9119360
  77. Gene fusions and RNA trans-splicing in normal and neoplastic human cells.
    Cell Cycle. 2009 Jan 15;8(2):218-22 PMID: 19158498
  78. Activation of an olfactory receptor inhibits proliferation of prostate cancer cells.
    J Biol Chem. 2009 Jun 12;284(24):16218-16225 PMID: 19389702
  79. Implications of chimaeric non-co-linear transcripts.
    Nature. 2009 Sep 10;461(7261):206-11 PMID: 19741701
  80. Identification of a prostate-specific G-protein coupled receptor in prostate cancer.
    Oncogene. 2001 Sep 13;20(41):5903-7 PMID: 11593396
  81. A putative pheromone receptor gene expressed in human olfactory mucosa.
    Nat Genet. 2000 Sep;26(1):18-9 PMID: 10973240
  82. Functional characterization of a mouse testicular olfactory receptor and its role in chemosensing and in regulation of sperm motility.
    J Cell Sci. 2004 Nov 15;117(Pt 24):5835-45 PMID: 15522887
Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0055368
Published
2013-00-00
Epub
2013-00-06
Pages
e55368
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3566163
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0055368 PMC Full Text View journal details
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