Home LiteratureArticle Details
PMID: 23878078 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Histone deacetylase AtHDA7 is required for female gametophyte and embryo development in Arabidopsis.

Plant physiology ·Vol. 163 ·No. 1 ·2013-09-00 ·Pages 431-40

Cigliano RA, Cremona G, Paparo R, Termolino P, Perrella G, Gutzat R, Consiglio MF, Conicella C

Abstract

Histone modifications are involved in the regulation of many processes in eukaryotic development. In this work, we provide evidence that AtHDA7, a HISTONE DEACETYLASE (HDAC) of the Reduced Potassium Dependency3 (RPD3) superfamily, is crucial for female gametophyte development and embryogenesis in Arabidopsis (Arabidopsis thaliana). Silencing of AtHDA7 causes degeneration of micropylar nuclei at the stage of four-nucleate embryo sac and delay in the progression of embryo development, thereby bringing the seed set down in the Athda7-2 mutant. Furthermore, AtHDA7 down- and up-regulation lead to a delay of growth in postgermination and later developmental stages. The Athda7-2 mutation that induces histone hyperacetylation significantly increases the transcription of other HDACs (AtHDA6 and AtHDA9). Moreover, silencing of AtHDA7 affects the expression of ARABIDOPSIS HOMOLOG OF SEPARASE (AtAESP), previously demonstrated to be involved in female gametophyte and embryo development. However, chromatin immunoprecipitation analysis with acetylated H3 antibody provided evidence that the acetylation levels of H3 at AtAESP and HDACs does not change in the mutant. Further investigations are essential to ascertain the mechanism by which AtHDA7 affects female gametophyte and embryo development.

MeSH Terms
Arabidopsis/enzymology,genetics,growth & development Arabidopsis Proteins/genetics,metabolism,physiology Gene Expression Regulation, Plant Gene Silencing Histone Deacetylases/genetics,metabolism,physiology Seeds/enzymology,genetics,growth & development
Chemicals
Arabidopsis Proteins Histone Deacetylases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Cigliano Riccardo Aiese
National Research Council of Italy, Institute of Plant Genetics, Research Division Portici, 80055 Portici, Italy.
Cremona Gaetana
Paparo Rosa
Termolino Pasquale
Perrella Giorgio
Gutzat Ruben
Consiglio Maria Federica
Conicella Clara
References (47)
47 references, click to expand
  1. Growth stage-based phenotypic analysis of Arabidopsis: a model for high throughput functional genomics in plants.
    Plant Cell. 2001 Jul;13(7):1499-510 PMID: 11449047
  2. Functional analysis of HD2 histone deacetylase homologues in Arabidopsis thaliana.
    Plant J. 2000 Apr;22(1):19-27 PMID: 10792817
  3. Genetic control of developmental changes induced by disruption of Arabidopsis histone deacetylase 1 (AtHD1) expression.
    Genetics. 2003 Sep;165(1):399-409 PMID: 14504245
  4. HDA18 affects cell fate in Arabidopsis root epidermis via histone acetylation at four kinase genes.
    Plant Cell. 2013 Jan;25(1):257-69 PMID: 23362208
  5. Prime, repair, restore: the active role of chromatin in the DNA damage response.
    Mol Cell. 2012 Jun 29;46(6):722-34 PMID: 22749398
  6. Leafy Cotyledon Mutants of Arabidopsis.
    Plant Cell. 1994 Aug;6(8):1049-1064 PMID: 12244265
  7. Determinants and dynamics of genome accessibility.
    Nat Rev Genet. 2011 Jul 12;12(8):554-64 PMID: 21747402
  8. Arabidopsis thaliana histone deacetylase 1 (AtHD1) is localized in euchromatic regions and demonstrates histone deacetylase activity in vitro.
    Cell Res. 2006 May;16(5):479-88 PMID: 16699543
  9. Different types of maize histone deacetylases are distinguished by a highly complex substrate and site specificity.
    Biochemistry. 1999 May 25;38(21):6769-73 PMID: 10346897
  10. TOPLESS regulates apical embryonic fate in Arabidopsis.
    Science. 2006 Jun 9;312(5779):1520-3 PMID: 16763149
  11. Involvement of Arabidopsis histone deacetylase HDA6 in ABA and salt stress response.
    J Exp Bot. 2010 Jul;61(12):3345-53 PMID: 20519338
  12. Alteration of GCN5 levels in maize reveals dynamic responses to manipulating histone acetylation.
    Plant J. 2003 Feb;33(3):455-69 PMID: 12581304
  13. Narrow A/T-rich zones present at the distal 5'-flanking sequences of the zein genes Zc1 and Zc2 bind a unique 30 kDa HMG-like protein.
    Plant Mol Biol. 1994 Dec;26(6):1893-906 PMID: 7858225
  14. The MYST histone acetyltransferases are essential for gametophyte development in Arabidopsis.
    BMC Plant Biol. 2008 Nov 28;8:121 PMID: 19040736
  15. Histone deacetylase genes in Arabidopsis development.
    J Integr Plant Biol. 2008 Jul;50(7):875-85 PMID: 18713398
  16. Reversible histone acetylation and deacetylation mediate genome-wide, promoter-dependent and locus-specific changes in gene expression during plant development.
    Genetics. 2005 Jan;169(1):337-45 PMID: 15371352
  17. Expression and function of HD2-type histone deacetylases in Arabidopsis development.
    Plant J. 2004 Jun;38(5):715-24 PMID: 15144374
  18. Floral spray transformation can efficiently generate Arabidopsis transgenic plants.
    Transgenic Res. 2000 Dec;9(6):471-6 PMID: 11206976
  19. HDA1 and RPD3 are members of distinct yeast histone deacetylase complexes that regulate silencing and transcription.
    Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14503-8 PMID: 8962081
  20. The radially swollen 4 separase mutation of Arabidopsis thaliana blocks chromosome disjunction and disrupts the radial microtubule system in meiocytes.
    PLoS One. 2011 Apr 29;6(4):e19459 PMID: 21559383
  21. Erasure of histone acetylation by Arabidopsis HDA6 mediates large-scale gene silencing in nucleolar dominance.
    Genes Dev. 2006 May 15;20(10):1283-93 PMID: 16648464
  22. Chromatin dynamics at DNA replication, transcription and repair.
    Eur J Biochem. 2004 Jun;271(12):2335-49 PMID: 15182349
  23. Identification of an enhancer/silencer sequence directing the aleurone-specific expression of a barley chitinase gene.
    Plant J. 1994 Oct;6(4):579-89 PMID: 7987416
  24. A gene expression map of Arabidopsis thaliana development.
    Nat Genet. 2005 May;37(5):501-6 PMID: 15806101
  25. Directed gene silencing with artificial microRNAs.
    Methods Mol Biol. 2010;592:71-88 PMID: 19802590
  26. Histone modifications in transcriptional activation during plant development.
    Biochim Biophys Acta. 2011 Oct;1809(10):567-76 PMID: 21777708
  27. Arabidopsis separase AESP is essential for embryo development and the release of cohesin during meiosis.
    Plant Cell. 2006 May;18(5):1213-25 PMID: 16582011
  28. Analysis of histone acetyltransferase and histone deacetylase families of Arabidopsis thaliana suggests functional diversification of chromatin modification among multicellular eukaryotes.
    Nucleic Acids Res. 2002 Dec 1;30(23):5036-55 PMID: 12466527
  29. HDA6, a putative histone deacetylase needed to enhance DNA methylation induced by double-stranded RNA.
    EMBO J. 2002 Dec 16;21(24):6832-41 PMID: 12486004
  30. The Arabidopsis histone deacetylases HDA6 and HDA19 contribute to the repression of embryonic properties after germination.
    Plant Physiol. 2008 Jan;146(1):149-61 PMID: 18024558
  31. Role of HD2 genes in seed germination and early seedling growth in Arabidopsis.
    Plant Cell Rep. 2011 Oct;30(10):1969-79 PMID: 21739146
  32. Genome-wide insertional mutagenesis of Arabidopsis thaliana.
    Science. 2003 Aug 1;301(5633):653-7 PMID: 12893945
  33. Early flower development in Arabidopsis.
    Plant Cell. 1990 Aug;2(8):755-67 PMID: 2152125
  34. Expression profile and cellular localization of maize Rpd3-type histone deacetylases during plant development.
    Plant Physiol. 2003 Oct;133(2):606-17 PMID: 12972660
  35. Reduced histone biosynthesis and chromatin changes arising from a damage signal at telomeres.
    Nat Struct Mol Biol. 2010 Oct;17(10):1218-25 PMID: 20890289
  36. Arabidopsis histone deacetylase HDA9 regulates flowering time through repression of AGL19.
    Biochem Biophys Res Commun. 2013 Mar 8;432(2):394-8 PMID: 23237803
  37. Silencer region of a chalcone synthase promoter contains multiple binding sites for a factor, SBF-1, closely related to GT-1.
    Plant Mol Biol. 1991 Feb;16(2):235-49 PMID: 1893099
  38. The WRKY superfamily of plant transcription factors.
    Trends Plant Sci. 2000 May;5(5):199-206 PMID: 10785665
  39. Nuclear behavior, cell polarity, and cell specification in the female gametophyte.
    Sex Plant Reprod. 2011 Jun;24(2):123-36 PMID: 21336612
  40. Female gametophytic mutants of Arabidopsis thaliana identified in a gene trap insertional mutagenesis screen.
    Int J Dev Biol. 2011;55(1):73-84 PMID: 21425082
  41. Dependence of heterochromatic histone H3 methylation patterns on the Arabidopsis gene DDM1.
    Science. 2002 Sep 13;297(5588):1871-3 PMID: 12077425
  42. Differential staining of aborted and nonaborted pollen.
    Stain Technol. 1969 May;44(3):117-22 PMID: 4181665
  43. RETINOBLASTOMA-RELATED PROTEIN controls the transition to autotrophic plant development.
    Development. 2011 Jul;138(14):2977-86 PMID: 21693514
  44. The Athb-1 and -2 HD-Zip domains homodimerize forming complexes of different DNA binding specificities.
    EMBO J. 1993 Sep;12(9):3507-17 PMID: 8253077
  45. Cis elements and trans-acting factors affecting regulation of a nonphotosynthetic light-regulated gene for chloroplast glutamine synthetase.
    Plant Physiol. 1995 Jul;108(3):1109-17 PMID: 7630938
  46. Histone hyperacetylation affects meiotic recombination and chromosome segregation in Arabidopsis.
    Plant J. 2010 Jun 1;62(5):796-806 PMID: 20230492
  47. Pds1 and Esp1 control both anaphase and mitotic exit in normal cells and after DNA damage.
    Genes Dev. 1999 Aug 1;13(15):1936-49 PMID: 10444592
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.113.221713
Published
2013-09-00
Epub
2013-00-22
Pages
431-40
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC3762662
Subset
IM
Grants
Austrian Science Fund FWF · I 489 · Austria
External Links
PubMed source DOI: 10.1104/pp.113.221713 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2