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PMID: 23893156 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation.

Journal of assisted reproduction and genetics ·Vol. 30 ·No. 9 ·2013-09-00 ·Pages 1187-202

Intasqui P, Camargo M, Del Giudice PT, Spaine DM, Carvalho VM, Cardozo KH, Cedenho AP, Bertolla RP

Abstract

Sperm DNA fragmentation has been suggested as a marker for infertility diagnosis and prognosis. Hence, understanding its impact on male physiology and post-genomic pathways would be clinically important. We performed the proteomics and functional enrichment analyses of viable spermatozoa from ejaculates with low and high sperm DNA fragmentation to identify protein expression and pathways altered in association with sperm DNA fragmentation. Sperm DNA fragmentation using the Comet assay and the Komet 6.0.1 software was assessed in raw samples from 89 subjects from a human reproduction service. The Low and High sperm DNA fragmentation groups were formed according to the Olive Tail Moment variable. Spermatozoa proteins from these groups were pooled and analyzed by a shotgun proteomic approach (2D nanoUPLC-ESI-MS(E)). Differentially expressed proteins were used for a functional enrichment study. Two hundred and fifty-seven proteins were identified or quantified in sperm from the Low and High sperm DNA fragmentation groups. Of these, seventy-one proteins were exclusively or overexpressed in the Low group, whereas twenty-three proteins were exclusively or overexpressed in the High group. One hundred and sixty-three proteins were conserved between these groups. We also functionally related the differentially expressed proteins in viable spermatozoa from the groups. Processes such as triacylglycerol metabolism, energy production, protein folding, response to unfolded proteins, and cellular detoxification were found to be altered in these cells. Sperm DNA fragmentation is associated with differential protein expression in viable spermatozoa. These proteins may potentially be used as biomarkers for sperm DNA integrity.

MeSH Terms
Cell Nucleus Comet Assay DNA/chemistry,genetics DNA Fragmentation Humans Male Metabolic Networks and Pathways/genetics Protein Biosynthesis Proteome Spermatozoa/chemistry,metabolism
Chemicals
Proteome DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Intasqui Paula
Department of Surgery, Division of Urology, Human Reproduction Section, Sao Paulo Federal University - Sao Paulo Hospital, R. Embau, 231, 04039-060, Sao Paulo, Brazil, [email protected].
Camargo Mariana
Del Giudice Paula T
Spaine Deborah M
Carvalho Valdemir M
Cardozo Karina H M
Cedenho Agnaldo P
Bertolla Ricardo P
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Article Info
Journal
Journal of assisted reproduction and genetics
Abbr.
J Assist Reprod Genet
ISSN
1573-7330
DOI
10.1007/s10815-013-0054-6
Email
[email protected]
Published
2013-09-00
Epub
2013-00-27
Pages
1187-202
Language
English
Region
Netherlands
NLM ID
9206495
PMCID
PMC3800528
Subset
IM
External Links
PubMed source DOI: 10.1007/s10815-013-0054-6 PMC Full Text View journal details
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